[English] 日本語
![](img/lk-miru.gif)
- PDB-1nnb: THREE-DIMENSIONAL STRUCTURE OF INFLUENZA A N9 NEURAMINIDASE AND I... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1nnb | ||||||
---|---|---|---|---|---|---|---|
Title | THREE-DIMENSIONAL STRUCTURE OF INFLUENZA A N9 NEURAMINIDASE AND ITS COMPLEX WITH THE INHIBITOR 2-DEOXY 2,3-DEHYDRO-N-ACETYL NEURAMINIC ACID | ||||||
![]() | NEURAMINIDASE | ||||||
![]() | HYDROLASE(O-GLYCOSYL) | ||||||
Function / homology | ![]() exo-alpha-(2->3)-sialidase activity / exo-alpha-(2->6)-sialidase activity / exo-alpha-(2->8)-sialidase activity / exo-alpha-sialidase / viral budding from plasma membrane / carbohydrate metabolic process / host cell plasma membrane / virion membrane / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Bossart-Whitaker, P. / Carson, M. / Babu, Y.S. / Smith, C.D. / Laver, W.G. / Air, G.M. | ||||||
![]() | ![]() Title: Three-dimensional structure of influenza A N9 neuraminidase and its complex with the inhibitor 2-deoxy 2,3-dehydro-N-acetyl neuraminic acid. Authors: Bossart-Whitaker, P. / Carson, M. / Babu, Y.S. / Smith, C.D. / Laver, W.G. / Air, G.M. #1: ![]() Title: Three-Dimensional Structure of Influenza A(Slash)Tern(Slash)Australia(Slash)G70C(Slash)75 N9 Neuraminidase and its Complex with the Inhibitor 2-Deoxy-2,3-Dehydro N-Acetyl Neuraminic Acid Authors: Bossart-Whitaker, P.J. #2: ![]() Title: Transfer of the Hemagglutinin Activity of Influenza Virus Neuraminidase Subtype N9 Into an N2 Background Authors: Nuss, J.M. / Air, G.M. #3: ![]() Title: Refined Atomic Structures of N9 Subtype Influenza Virus Neuraminidase and Escape Mutants Authors: Tulip, W.R. / Varghese, J.N. / Baker, A.T. / Van Donkelaar, A. / Laver, W.G. / Webster, R.G. / Colman, P.M. #4: ![]() Title: Three-Dimensional Structure of Neuraminidase of Subtype N9 from an Avian Influenza Virus Authors: Baker, A.T. / Varghese, J.N. / Laver, W.G. / Air, G.M. / Colman, P.M. #5: ![]() Title: Gene and Protein Sequence of an Influenza Neuraminidase with Hemagglutinin Activity Authors: Air, G.M. / Ritchie, L.R. / Laver, W.G. / Colman, P.M. #6: ![]() Title: Influenza Virus Neuraminidase with Hemagglutinin Activity Authors: Laver, W.G. / Colman, P.M. / Webster, R.G. / Hinshaw, V.S. #7: ![]() Title: Memorandum: A Revision of the System of Nomenclature for Influenza Viruses | ||||||
History |
| ||||||
Remark 700 | SHEET STRAND 1 OF SHEET S3 (LEU 225 - HIS 235) IS NOT REALLY PART OF THE SHEET BY H-BONDING ...SHEET STRAND 1 OF SHEET S3 (LEU 225 - HIS 235) IS NOT REALLY PART OF THE SHEET BY H-BONDING CRITERIA BECAUSE IT IS IN AN EXTENDED CONFORMATION. OTHERS HAVE BEEN INCLUDED IN THE SHEET MOSTLY FOR THE SAKE OF SYMMETRY; I.E., THE STRUCTURE THEN CONSISTS OF SIX SHEETS OF FOUR ANTIPARALLEL STRANDS EACH. |
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 99 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 80.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 455.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 465.7 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 14.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: CIS PROLINE - PRO 328 / 2: CIS PROLINE - PRO 433 |
-
Components
#1: Protein | Mass: 43566.578 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
---|---|
#2: Chemical | ChemComp-CA / |
#3: Sugar | ChemComp-DAN / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.54 % |
---|---|
Crystal grow | *PLUS pH: 6.6 / Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Conc.: 1.7 M / Common name: potassium phosphate |
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.8→6 Å Details: RESIDUE 336 IS GLY. IT WAS REFINED AS ASN AND THE AUTHORS NOTED THAT THE SIDE CHAIN OF THIS RESIDUE NEVER DISPLAYED DENSITY (SEE FIGURE 3A OF THE PAPER CITED ON *JRNL* RECORDS ABOVE). THE ...Details: RESIDUE 336 IS GLY. IT WAS REFINED AS ASN AND THE AUTHORS NOTED THAT THE SIDE CHAIN OF THIS RESIDUE NEVER DISPLAYED DENSITY (SEE FIGURE 3A OF THE PAPER CITED ON *JRNL* RECORDS ABOVE). THE AUTHORS BELIEVE THAT CHANGING IT TO GLY WILL HAVE A NEGLIGIBLE EFFECT ON THE REST OF THE STRUCTURE.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→6 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.179 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.6 |