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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1nfi | ||||||
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タイトル | I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX | ||||||
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![]() | COMPLEX (TRANSCRIPTION REG/ANK REPEAT) / COMPLEX (TRANSCRIPTION REGULATION-ANK REPEAT) / ANKYRIN REPEAT / COMPLEX (TRANSCRIPTION REG-ANK REPEAT) COMPLEX | ||||||
機能・相同性 | ![]() negative regulation of calcidiol 1-monooxygenase activity / I-kappaB/NF-kappaB complex / negative regulation of vitamin D biosynthetic process / negative regulation of cholesterol transport / positive regulation of hyaluronan biosynthetic process / cytoplasmic sequestering of NF-kappaB / negative regulation of myeloid cell differentiation / antibacterial innate immune response / mammary gland involution / acetaldehyde metabolic process ...negative regulation of calcidiol 1-monooxygenase activity / I-kappaB/NF-kappaB complex / negative regulation of vitamin D biosynthetic process / negative regulation of cholesterol transport / positive regulation of hyaluronan biosynthetic process / cytoplasmic sequestering of NF-kappaB / negative regulation of myeloid cell differentiation / antibacterial innate immune response / mammary gland involution / acetaldehyde metabolic process / prolactin signaling pathway / cellular response to interleukin-17 / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / NF-kappaB p50/p65 complex / nucleotide-binding oligomerization domain containing 1 signaling pathway / IkBA variant leads to EDA-ID / positive regulation of macrophage derived foam cell differentiation / positive regulation of Schwann cell differentiation / positive regulation of lipid storage / cellular response to peptidoglycan / molecular sequestering activity / negative regulation of interleukin-12 production / ankyrin repeat binding / RIP-mediated NFkB activation via ZBP1 / Regulated proteolysis of p75NTR / SUMOylation of immune response proteins / CLEC7A/inflammasome pathway / negative regulation of protein sumoylation / defense response to tumor cell / postsynapse to nucleus signaling pathway / nucleotide-binding oligomerization domain containing 2 signaling pathway / cellular response to interleukin-6 / Interleukin-1 processing / cellular response to dsRNA / negative regulation of non-canonical NF-kappaB signal transduction / actinin binding / cellular response to angiotensin / transcription regulator inhibitor activity / response to UV-B / NF-kappaB complex / negative regulation of protein metabolic process / positive regulation of leukocyte adhesion to vascular endothelial cell / cellular response to cold / Regulation of NFE2L2 gene expression / interleukin-1-mediated signaling pathway / vascular endothelial growth factor signaling pathway / positive regulation of miRNA metabolic process / non-canonical NF-kappaB signal transduction / toll-like receptor 4 signaling pathway / nuclear localization sequence binding / negative regulation of NF-kappaB transcription factor activity / cellular response to hepatocyte growth factor stimulus / positive regulation of amyloid-beta formation / response to cobalamin / negative regulation of Notch signaling pathway / positive regulation of T cell receptor signaling pathway / response to exogenous dsRNA / phosphate ion binding / cellular response to lipoteichoic acid / response to muramyl dipeptide / TRAF6 mediated NF-kB activation / negative regulation of macrophage derived foam cell differentiation / The NLRP3 inflammasome / negative regulation of lipid storage / general transcription initiation factor binding / neuropeptide signaling pathway / Transcriptional Regulation by VENTX / NF-kappaB binding / hair follicle development / cellular response to interleukin-1 / positive regulation of vascular endothelial growth factor production / positive regulation of cholesterol efflux / positive regulation of transcription initiation by RNA polymerase II / response to amino acid / RNA polymerase II core promoter sequence-specific DNA binding / cellular defense response / Purinergic signaling in leishmaniasis infection / canonical NF-kappaB signal transduction / negative regulation of canonical NF-kappaB signal transduction / antiviral innate immune response / JNK cascade / lipopolysaccharide-mediated signaling pathway / negative regulation of insulin receptor signaling pathway / Notch signaling pathway / tumor necrosis factor-mediated signaling pathway / response to cAMP / protein sequestering activity / response to muscle stretch / positive regulation of protein metabolic process / positive regulation of interleukin-12 production / CD209 (DC-SIGN) signaling / NF-kB is activated and signals survival / response to interleukin-1 / negative regulation of angiogenesis / MAP3K8 (TPL2)-dependent MAPK1/3 activation / negative regulation of miRNA transcription / liver development / positive regulation of interleukin-1 beta production / response to cytokine / positive regulation of interleukin-8 production 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Jacobs, M.D. / Harrison, S.C. | ||||||
![]() | ![]() タイトル: Structure of an IkappaBalpha/NF-kappaB complex. 著者: Jacobs, M.D. / Harrison, S.C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 249.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 200.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (-1, 0.003, -0.001), ベクター: |
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要素
#1: タンパク質 | 分子量: 34547.199 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質 | 分子量: 12442.117 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #3: タンパク質 | 分子量: 23312.512 Da / 分子数: 2 / Fragment: ANKYRIN / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #4: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.02 Å3/Da / 溶媒含有率: 59 % | |||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 5 / 詳細: pH 5.0 | |||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 4, 20 ℃ / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 110 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: BRANDEIS - B4 / 検出器: CCD |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9879 Å / 相対比: 1 |
反射 | 解像度: 2.7→20 Å / Num. obs: 41990 / % possible obs: 92 % / 冗長度: 3.6 % / Biso Wilson estimate: 60.4 Å2 / Rsym value: 0.05 |
反射 | *PLUS 冗長度: 3.7 % / Rmerge(I) obs: 0.05 |
反射 シェル | *PLUS % possible obs: 83 % / Rmerge(I) obs: 0.206 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]()
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 37.18 Å2 / ksol: 0.343 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 59.8 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.7→20 Å
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拘束条件 |
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Refine LS restraints NCS | NCS model details: CONSTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS精密化 シェル | 解像度: 2.7→2.8 Å / Rfactor Rfree error: 0.028 / Total num. of bins used: 10
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Xplor file |
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ソフトウェア | *PLUS 名称: CNS / バージョン: 0.5 / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Num. reflection obs: 39742 / Rfactor obs: 0.222 / Rfactor Rfree: 0.315 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor obs: 0.315 |