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Yorodumi- PDB-1nf3: Structure of Cdc42 in a complex with the GTPase-binding domain of... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1nf3 | ||||||
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Title | Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6 | ||||||
Components |
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Keywords | SIGNALING PROTEIN / semi-CRIB motif / Switch I and II / PDZ domain / GTPase binding domain | ||||||
Function / homology | Function and homology information Tight junction interactions / RHOV GTPase cycle / regulation of cellular localization / GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure / endothelin receptor signaling pathway involved in heart process ...Tight junction interactions / RHOV GTPase cycle / regulation of cellular localization / GBD domain binding / submandibular salivary gland formation / actin filament branching / Golgi transport complex / positive regulation of pinocytosis / modification of synaptic structure / endothelin receptor signaling pathway involved in heart process / Cdc42 protein signal transduction / cardiac neural crest cell migration involved in outflow tract morphogenesis / positive regulation of synapse structural plasticity / dendritic cell migration / storage vacuole / apolipoprotein A-I receptor binding / positive regulation of epithelial cell proliferation involved in lung morphogenesis / neuron fate determination / modulation by host of viral process / organelle transport along microtubule / regulation of attachment of spindle microtubules to kinetochore / positive regulation of pseudopodium assembly / Inactivation of CDC42 and RAC1 / cardiac conduction system development / GTP-dependent protein binding / regulation of filopodium assembly / establishment of Golgi localization / leading edge membrane / neuropilin signaling pathway / positive regulation of intracellular protein transport / cell junction assembly / filopodium assembly / establishment of epithelial cell apical/basal polarity / regulation of modification of postsynaptic structure / dendritic spine morphogenesis / mitogen-activated protein kinase kinase kinase binding / embryonic heart tube development / thioesterase binding / regulation of stress fiber assembly / RHO GTPases activate KTN1 / regulation of lamellipodium assembly / nuclear migration / DCC mediated attractive signaling / adherens junction organization / centrosome cycle / sprouting angiogenesis / Wnt signaling pathway, planar cell polarity pathway / CD28 dependent Vav1 pathway / regulation of postsynapse organization / positive regulation of filopodium assembly / regulation of mitotic nuclear division / establishment or maintenance of cell polarity / phagocytosis, engulfment / RHOV GTPase cycle / heart contraction / Myogenesis / RHOJ GTPase cycle / Golgi organization / RHOQ GTPase cycle / positive regulation of cytokinesis / RHO GTPases activate PAKs / CDC42 GTPase cycle / RHOU GTPase cycle / macrophage differentiation / RHOG GTPase cycle / bicellular tight junction / RHO GTPases Activate WASPs and WAVEs / RAC3 GTPase cycle / RAC2 GTPase cycle / RHO GTPases activate IQGAPs / spindle midzone / positive regulation of DNA replication / negative regulation of protein-containing complex assembly / phagocytic vesicle / positive regulation of lamellipodium assembly / positive regulation of substrate adhesion-dependent cell spreading / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / RAC1 GTPase cycle / EPHB-mediated forward signaling / substantia nigra development / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / small monomeric GTPase / G protein activity / filopodium / secretory granule / actin filament organization / integrin-mediated signaling pathway / RHO GTPases Activate Formins / regulation of actin cytoskeleton organization / FCGR3A-mediated phagocytosis / EGFR downregulation / positive regulation of JNK cascade / MAPK6/MAPK4 signaling / Schaffer collateral - CA1 synapse / protein localization / G beta:gamma signalling through CDC42 / cytoplasmic ribonucleoprotein granule / mitotic spindle / Regulation of actin dynamics for phagocytic cup formation Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Garrard, S.M. / Capaldo, C.T. / Gao, L. / Rosen, M.K. / Macara, I.G. / Tomchick, D.R. | ||||||
Citation | Journal: Embo J. / Year: 2003 Title: Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6 Authors: Garrard, S.M. / Capaldo, C.T. / Gao, L. / Rosen, M.K. / Macara, I.G. / Tomchick, D.R. #1: Journal: Curr.Biol. / Year: 2002 Title: Assembly of epithelial tight junctions is negatively regulated by Par6 Authors: Gao, L. / Joberty, G. / Macara, I.G. #2: Journal: Nat.Cell Biol. / Year: 2000 Title: The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42 Authors: Joberty, G. / Petersen, C. / Gao, L. / Macara, I.G. #3: Journal: Nat.Cell Biol. / Year: 2000 Title: A mammalian Par-3-Par-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity Authors: Lin, D. / Edwards, A.S. / Fawcett, J.P. / Mbamalu, G. / Scott, J.D. / Pawson, T. #4: Journal: Curr.Biol. / Year: 2000 Title: A human homolog of the C. elegans polarity determinant Par-6 links Rac and Cdc42 to PKCzeta signaling and cell transformation Authors: Qiu, R.G. / Abo, A. / Steven Martin, G. #5: Journal: DEVELOPMENT / Year: 1996 Title: Par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of Par-3 Authors: Watts, J.L. / Etemad-Moghadam, B. / Guo, S. / Boyd, L. / Draper, B.W. / Mello, C.C. / Priess, J.R. / Kemphues, K.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nf3.cif.gz | 147.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nf3.ent.gz | 113.5 KB | Display | PDB format |
PDBx/mmJSON format | 1nf3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nf/1nf3 ftp://data.pdbj.org/pub/pdb/validation_reports/nf/1nf3 | HTTPS FTP |
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-Related structure data
Related structure data | 2ngrS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 21566.949 Da / Num. of mol.: 2 / Mutation: Q61L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CDC42 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P60953 #2: Protein | Mass: 14175.417 Da / Num. of mol.: 2 / Fragment: GTPase-binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Par6B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9JK83 #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.46 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.4 Details: 24% PEG 4000, 100mM sodium citrate, 0.2M ammonium acetate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 8.5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9184 Å |
Detector | Type: SBC-2 / Detector: CCD / Date: Mar 8, 2002 / Details: double crystal monochromator |
Radiation | Monochromator: graphite double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→40.5 Å / Num. all: 38985 / Num. obs: 38985 / % possible obs: 89.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2 % / Biso Wilson estimate: 14.7 Å2 / Rmerge(I) obs: 0.104 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 2.1→2.14 Å / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2 / % possible all: 87.1 |
Reflection | *PLUS Num. measured all: 80223 |
Reflection shell | *PLUS % possible obs: 87.1 % / Rmerge(I) obs: 0.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2NGR Resolution: 2.1→29.17 Å / Rfactor Rfree error: 0.007 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 46.2756 Å2 / ksol: 0.35546 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→29.17 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.18 Å / Rfactor Rfree error: 0.024 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 30 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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