+Open data
-Basic information
Entry | Database: PDB / ID: 1nbb | ||||||
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Title | N-BUTYLISOCYANIDE BOUND RHODOBACTER CAPSULATUS CYTOCHROME C' | ||||||
Components | CYTOCHROME C' | ||||||
Keywords | ELECTRON TRANSPORT (HEME PROTEIN) / ELECTRON TRANSPORT / CYTOCHROME / HEME PROTEIN | ||||||
Function / homology | Function and homology information electron transport chain / periplasmic space / electron transfer activity / iron ion binding / heme binding Similarity search - Function | ||||||
Biological species | Rhodobacter capsulatus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Tahirov, T.H. / Misaki, S. / Meyer, T.E. / Cusanovich, M.A. / Higuchi, Y. / Yasuoka, N. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: Concerted movement of side chains in the haem vicinity observed on ligand binding in cytochrome c' from rhodobacter capsulatus. Authors: Tahirov, T.H. / Misaki, S. / Meyer, T.E. / Cusanovich, M.A. / Higuchi, Y. / Yasuoka, N. #1: Journal: To be Published Title: Crystal Structures of Cytochrome C' from Rhodobacter Capsulatus Strain St. Louis Authors: Tahirov, T.H. / Misaki, S. / Meyer, T.E. / Cusanovich, M.A. / Yasuoka, N. #2: Journal: J.Mol.Biol. / Year: 1996 Title: High-Resolution Crystal Structures of Two Polymorphs of Cytochrome C' from the Purple Phototrophic Bacterium Rhodobacter Capsulatus Authors: Tahirov, T.H. / Misaki, S. / Meyer, T.E. / Cusanovich, M.A. / Higuchi, Y. / Yasuoka, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nbb.cif.gz | 61.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nbb.ent.gz | 45.8 KB | Display | PDB format |
PDBx/mmJSON format | 1nbb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1nbb_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 1nbb_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 1nbb_validation.xml.gz | 14.3 KB | Display | |
Data in CIF | 1nbb_validation.cif.gz | 18.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nb/1nbb ftp://data.pdbj.org/pub/pdb/validation_reports/nb/1nbb | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13154.733 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: N-BUTYLISOCYANIDE BOUND TO HEME IRON / Source: (natural) Rhodobacter capsulatus (bacteria) / Strain: M110 / References: UniProt: P00147 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 53 % | |||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | |||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop / Details: Tahirov, T.H., (1996) J.Mol.Biol., 259, 467. | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 283 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Detector: IMAGE PLATE / Date: Sep 15, 1995 / Details: DOUBLE MIRROR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 49034 / % possible obs: 92 % / Observed criterion σ(I): 1 / Redundancy: 4.44 % / Rmerge(I) obs: 0.129 / Net I/σ(I): 9.28 |
Reflection shell | Resolution: 2.4→2.49 Å / Mean I/σ(I) obs: 3.1 / % possible all: 73 |
Reflection | *PLUS Num. obs: 11056 / Num. measured all: 49034 |
Reflection shell | *PLUS % possible obs: 73 % / Rmerge(I) obs: 0.261 |
-Processing
Software |
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Refinement | Resolution: 2.4→6 Å / σ(F): 1
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Displacement parameters | Biso mean: 29.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 2.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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