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- PDB-1n49: Viability of a Drug-Resistant HIV-1 Protease Variant: Structural ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1n49 | ||||||
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Title | Viability of a Drug-Resistant HIV-1 Protease Variant: Structural Insights for Better Anti-Viral Therapy | ||||||
![]() | Protease | ||||||
![]() | HYDROLASE/HYDROLASE INHIBITOR / HIV-1 protease / drug resistance / substrate recognition / inhibitor binding / HYDROLASE / HYDROLASE-HYDROLASE INHIBITOR complex | ||||||
Function / homology | ![]() HIV-1 retropepsin / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus ...HIV-1 retropepsin / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / host cell / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / symbiont-mediated suppression of host gene expression / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / proteolysis / DNA binding / zinc ion binding / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Prabu-Jeyabalan, M. / Nalivaika, E.A. / King, N.M. / Schiffer, C.A. | ||||||
![]() | ![]() Title: Viability of a Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variant: Structural Insights for Better Antiviral Therapy Authors: Prabu-Jeyabalan, M. / Nalivaika, E.A. / King, N.M. / Schiffer, C.A. #1: ![]() Title: How does a symmetric dimer recognize an asymmetric substrate? a substrate complex of HIV-1 protease Authors: Prabu-Jeyabalan, M. / Nalivaika, E. / Schiffer, C.A. #2: ![]() Title: Substrate shape determines specificity of recognition for HIV-1 protease: analysis of crystal structures of six substrate complexes Authors: Prabu-Jeyabalan, M. / Nalivaika, E. / Schiffer, C.A. #3: ![]() Title: Lack of synergy for inhibitors targeting a multi-drug resistant HIV-1 protease Authors: King, N.M. / Melnick, L. / Prabu-Jeyabalan, M. / Nalivaika, E.A. / Yang, S.S. / Gao, Y. / Nie, X. / Zepp, C. / Heefner, D.L. / Schiffer, C.A. #4: ![]() Title: ABT-538 Is a Potent Inhibitor of Human Immunodeficiency Virus Protease and has High Oral Bioavailability in Humans Authors: Kempf, D.J. / Marsh, K.C. / Denissen, J.F. / McDonald, E. / Vasavanonda, S. / Flentge, C.A. / Green, B.E. / Fino, L. / Park, C.H. / Kong, X. / Wideburg, N.E. / Saldivar, A. / Ruiz, L. / ...Authors: Kempf, D.J. / Marsh, K.C. / Denissen, J.F. / McDonald, E. / Vasavanonda, S. / Flentge, C.A. / Green, B.E. / Fino, L. / Park, C.H. / Kong, X. / Wideburg, N.E. / Saldivar, A. / Ruiz, L. / Kati, W.M. / Sham, H.L. / Robins, T. / Stewart, K.D. / Hsu, A. / Plattner, J.J. / Leonard, J.M. / Norbeck, D.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.2 KB | Display | ![]() |
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PDB format | ![]() | 65.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 559.3 KB | Display | ![]() |
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Full document | ![]() | 578.9 KB | Display | |
Data in XML | ![]() | 12.6 KB | Display | |
Data in CIF | ![]() | 17.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mt7C ![]() 1mt8C ![]() 1mt9C ![]() 1mtbC ![]() 1f7aS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10772.724 Da / Num. of mol.: 4 / Mutation: Q7K, D25N, V82A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.59 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6.2 Details: sodium phosphate, sodium citrate, ammonium sulphate, pH 6.2, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 200 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Aug 7, 2001 / Details: Yale Mirrors |
Radiation | Monochromator: Yale Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 13097 / Num. obs: 13097 / % possible obs: 71.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.064 / Net I/σ(I): 7.6 |
Reflection shell | Resolution: 2.2→2.28 Å / Rmerge(I) obs: 0.27 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. measured all: 19193 |
Reflection shell | *PLUS % possible obs: 66 % / Num. unique obs: 1184 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1F7A Resolution: 2.2→50 Å / Isotropic thermal model: restrained / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: NCS RESTRAINTS IMPOSED BETWEEN THE DIMERS (BUT NOT WITHIN THE DIMERS) TO IMPROVE OBSERVABLES TO PARAMETER RATIO.
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.28 Å
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Refinement | *PLUS Lowest resolution: 50 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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