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- PDB-1myk: CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTAB... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1myk | ||||||
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Title | CRYSTAL STRUCTURE, FOLDING, AND OPERATOR BINDING OF THE HYPERSTABLE ARC REPRESSOR MUTANT PL8 | ||||||
![]() | ARC REPRESSOR | ||||||
![]() | TRANSCRIPTION REGULATION | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Schildbach, J.F. / Milla, M.E. / Jeffrey, P.D. / Raumann, B.E. / Sauer, R.T. | ||||||
![]() | ![]() Title: Crystal structure, folding, and operator binding of the hyperstable Arc repressor mutant PL8. Authors: Schildbach, J.F. / Milla, M.E. / Jeffrey, P.D. / Raumann, B.E. / Sauer, R.T. #1: ![]() Title: DNA Recognition by Beta-Sheets in the Arc Repressor-Operator Crystal Structure Authors: Raumann, B.E. / Rould, M.A. / Pabo, C.O. / Sauer, R.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28.9 KB | Display | ![]() |
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PDB format | ![]() | 19.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 376.3 KB | Display | ![]() |
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Full document | ![]() | 378.7 KB | Display | |
Data in XML | ![]() | 3.6 KB | Display | |
Data in CIF | ![]() | 4.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 6254.315 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | Compound details | PL8 IS HYPERSTABLE RELATIVE TO ARC, BUT HAS REDUCED AFFINITY FOR DNA. PL8 DIMER IS 2.5 KCALMOL MORE ...PL8 IS HYPERSTABL | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.56 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 5340 / % possible obs: 90.4 % / Observed criterion σ(I): 0 |
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 6 Å / Rmerge(I) obs: 0.094 |
Reflection shell | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 2.5 Å / % possible obs: 56.6 % |
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Processing
Software |
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Refinement | Resolution: 2.4→6 Å / σ(F): 0
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Displacement parameters | Biso mean: 33.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→6 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection all: 5340 / Rfactor obs: 0.217 / Rfactor Rfree: 0.277 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.49 |