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Yorodumi- PDB-1mux: SOLUTION NMR STRUCTURE OF CALMODULIN/W-7 COMPLEX: THE BASIS OF DI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1mux | ||||||
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Title | SOLUTION NMR STRUCTURE OF CALMODULIN/W-7 COMPLEX: THE BASIS OF DIVERSITY IN MOLECULAR RECOGNITION, 30 STRUCTURES | ||||||
Components | CALMODULIN | ||||||
Keywords | CALCIUM-BINDING / COMPLEX (CALMODULIN-INHIBITOR) / CALMODULIN / W-7 / NAPHTHALENESULFONAMIDE | ||||||
Function / homology | Function and homology information enzyme regulator activity / signaling receptor binding / calcium ion binding Similarity search - Function | ||||||
Biological species | Xenopus laevis (African clawed frog) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Osawa, M. / Swindells, M.B. / Tanikawa, J. / Tanaka, T. / Mase, T. / Furuya, T. / Ikura, M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1998 Title: Solution structure of calmodulin-W-7 complex: the basis of diversity in molecular recognition. Authors: Osawa, M. / Swindells, M.B. / Tanikawa, J. / Tanaka, T. / Mase, T. / Furuya, T. / Ikura, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mux.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1mux.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 1mux.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mux_validation.pdf.gz | 477.5 KB | Display | wwPDB validaton report |
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Full document | 1mux_full_validation.pdf.gz | 727.2 KB | Display | |
Data in XML | 1mux_validation.xml.gz | 132 KB | Display | |
Data in CIF | 1mux_validation.cif.gz | 154.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mu/1mux ftp://data.pdbj.org/pub/pdb/validation_reports/mu/1mux | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16721.350 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xenopus laevis (African clawed frog) / Plasmid: PAS / Production host: Escherichia coli (E. coli) / References: UniProt: P62155, UniProt: P0DP33*PLUS | ||
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#2: Chemical | ChemComp-CA / #3: Chemical | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: DUE TO THE LACK OF NOE'S BETWEEN THE N- AND C- TERMINAL DOMAINS, IT IS IMPOSSIBLE TO SUPERIMPOSE BOTH DOMAINS OF THE 30 NMR-DERIVED STRUCTURES SIMULTANEOOUSLY. HENCE, THE BACKBONE ATOMS OF THE ...Text: DUE TO THE LACK OF NOE'S BETWEEN THE N- AND C- TERMINAL DOMAINS, IT IS IMPOSSIBLE TO SUPERIMPOSE BOTH DOMAINS OF THE 30 NMR-DERIVED STRUCTURES SIMULTANEOOUSLY. HENCE, THE BACKBONE ATOMS OF THE C-TERMINAL DOMAIN HAVE HAVE BEEN SUPERIMPOSED. |
-Sample preparation
Sample conditions | Ionic strength: KCL, 100mM / pH: 6.8 / Pressure: 1 atm / Temperature: 308 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX 600 / Manufacturer: Bruker / Model: AMX 600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformers calculated total number: 100 / Conformers submitted total number: 30 |