+
Open data
-
Basic information
Entry | Database: PDB / ID: 1mrp | ||||||
---|---|---|---|---|---|---|---|
Title | FERRIC-BINDING PROTEIN FROM HAEMOPHILUS INFLUENZAE | ||||||
![]() | FERRIC IRON BINDING PROTEIN | ||||||
![]() | IRON TRANSPORT / FERRIC IRON BINDING PROTEIN / PERIPLASMIC BINDING PROTEIN / TRANSFERRIN SUPERFAMILY | ||||||
Function / homology | ![]() transmembrane transport / iron ion transport / outer membrane-bounded periplasmic space / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bruns, C.M. / Nowalk, A.J. / Arvai, A.S. / Mctigue, M.A. / Vaughan, K.G. / Mietzner, T.A. / Mcree, D.E. | ||||||
![]() | ![]() Title: Structure of Haemophilus influenzae Fe(+3)-binding protein reveals convergent evolution within a superfamily. Authors: Bruns, C.M. / Nowalk, A.J. / Arvai, A.S. / McTigue, M.A. / Vaughan, K.G. / Mietzner, T.A. / McRee, D.E. #1: ![]() Title: Biochemical Characterization of a Haemophilus Influenzae Periplasmic Iron Transport Operon Authors: Adhikari, P. / Kirby, S.D. / Nowalk, A.J. / Veraldi, K.L. / Schryvers, A.B. / Mietzner, T.A. #2: ![]() Title: Coordination of Iron by the Ferric Iron-Binding Protein of Pathogenic Neisseria is Homologous to the Transferrins Authors: Nowalk, A.J. / Tencza, S.B. / Mietzner, T.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 76.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 57.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 429.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 437.1 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 22.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 33769.250 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-FE / |
#3: Chemical | ChemComp-PO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 35 % | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 6.6 Details: 20% PEG 4000, 400 MM IMIDAZOLE/MALATE PH 6.6, 200 MM NACL | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 292 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: MIRRORS / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→50 Å / Num. obs: 36442 / % possible obs: 93 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rsym value: 0.072 / Net I/σ(I): 27.3 |
Reflection shell | Resolution: 1.6→1.66 Å / Redundancy: 1.54 % / Mean I/σ(I) obs: 2.1 / Rsym value: 0.282 / % possible all: 54 |
Reflection | *PLUS Rmerge(I) obs: 0.072 |
Reflection shell | *PLUS % possible obs: 54 % / Rmerge(I) obs: 0.282 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Cross valid method: THROUGHOUT, EXCEPT FINAL ROUND OF REFINEMENT σ(F): 0 / Stereochemistry target values: TNT PROTGEO
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: BABINET SCALING / Bsol: 182.5 Å2 / ksol: 0.79 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→50 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: TNT / Version: 5E / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.175 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
| ||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 1.6 Å / Lowest resolution: 1.66 Å / Rfactor all: 0.36 |