+
Open data
-
Basic information
Entry | Database: PDB / ID: 1mn4 | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of Ndt80 (Residues 59-340) DNA-binding domain core | ||||||
![]() | NDT80 PROTEIN | ||||||
![]() | TRANSCRIPTION / IG FOLD / PROTEIN-DNA COMPLEX | ||||||
Function / homology | ![]() sporulation / nuclear chromosome / meiotic cell cycle / sequence-specific DNA binding / DNA-binding transcription factor activity / cell division / positive regulation of transcription by RNA polymerase II Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lamoureux, J.S. / Stuart, D. / Tsang, R. / Wu, C. / Glover, J.N.M. | ||||||
![]() | ![]() Title: Structure of the sporulation-specific transcription factor Ndt80 bound to DNA Authors: Lamoureux, J.S. / Stuart, D. / Tsang, R. / Wu, C. / Glover, J.N.M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 64.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 46.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 32385.701 Da / Num. of mol.: 1 / Fragment: DNA-binding core (Residues 59-340) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: NDT80 / Plasmid: pGEX-6P1 / Species (production host): Escherichia coli / Production host: ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.91 Å3/Da / Density % sol: 35.58 % | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 4000, sodium acetate, magnesium chloride, spermine, 20mer DNA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 5.5 | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 105 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 21, 2002 / Details: bent cylindrical Si-mirror (Rh coating) |
Radiation | Monochromator: Si(111) double-crystal / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9563 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→14.95 Å / Num. all: 12530 / Num. obs: 13292 / % possible obs: 98.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 11.7 % / Biso Wilson estimate: 14.3 Å2 / Rsym value: 0.076 / Net I/σ(I): 20.9 |
Reflection shell | Resolution: 2.2→2.25 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 7.9 / Rsym value: 0.221 / % possible all: 100 |
Reflection | *PLUS Lowest resolution: 40 Å / Num. obs: 12292 / Num. measured all: 144249 / Rmerge(I) obs: 0.076 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.221 |
-
Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||||||||||
Displacement parameters |
| ||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.27 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.21 Å | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→14.95 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
Xplor file |
| ||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 40 Å / Rfactor all: 0.229 / Rfactor Rfree: 0.268 / Rfactor Rwork: 0.226 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.28 |