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- PDB-1mjh: Structure-based assignment of the biochemical function of hypothe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mjh | ||||||
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Title | Structure-based assignment of the biochemical function of hypothetical protein MJ0577: A test case of structural genomics | ||||||
![]() | PROTEIN (ATP-BINDING DOMAIN OF PROTEIN MJ0577) | ||||||
![]() | HYPOTHETICAL PROTEIN / STRUCTURAL GENOMICS / FUNCTIONAL ASSIGNMENT / ATP BINDING PROTEIN / BSGC structure funded by NIH / Protein Structure Initiative / PSI / Berkeley Structural Genomics Center | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zarembinski, T.I. / Hung, L.-W. / Mueller-Dieckmann, H.J. / Kim, K.-K. / Yokota, H. / Kim, R. / Kim, S.-H. / Berkeley Structural Genomics Center (BSGC) | ||||||
![]() | ![]() Title: Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics. Authors: Zarembinski, T.I. / Hung, L.-W. / Mueller-Dieckmann, H.J. / Kim, K.-K. / Yokota, H. / Kim, R. / Kim, S.-H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79 KB | Display | ![]() |
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PDB format | ![]() | 58.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 17.2 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18370.600 Da / Num. of mol.: 2 / Fragment: ATP-BINDING DOMAIN Source method: isolated from a genetically manipulated source Details: COMPLEXED WITH ADENOSINE-5'-TRIPHOSPHATE Source: (gene. exp.) ![]() ![]() Description: RECENTLY SEQUENCED HYPERTHERMOPHILE / Cellular location: CYTOPLASM / Gene: MJ0577 / Plasmid: PET-23A / Production host: ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.48 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 / Details: pH 7.0, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() ![]() ![]() | |||||||||||||||
Detector | Detector: CCD / Date: Mar 15, 1998 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→20 Å / Num. obs: 38706 / % possible obs: 98 % / Redundancy: 7.5 % / Rsym value: 0.049 | |||||||||||||||
Reflection shell | Highest resolution: 1.7 Å / Rsym value: 0.0249 / % possible all: 85.4 | |||||||||||||||
Reflection | *PLUS Num. measured all: 292156 / Rmerge(I) obs: 0.049 | |||||||||||||||
Reflection shell | *PLUS % possible obs: 85.4 % / Rmerge(I) obs: 0.249 |
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Processing
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Refinement | Method to determine structure: ![]() Details: REFINEMENT WAS BEGUN WITH CNS AND FINAL REFINEMENT WITH REFMAC AND ARP
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Displacement parameters | Biso mean: 30.1 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.21 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |