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- PDB-1b8x: GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1b8x | ||||||
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Title | GLUTATHIONE S-TRANSFERASE FUSED WITH THE NUCLEAR MATRIX TARGETING SIGNAL OF THE TRANSCRIPTION FACTOR AML-1 | ||||||
![]() | PROTEIN (AML-1B) | ||||||
![]() | SIGNAL PROTEIN / NUCLEAR MATRIX TARGETING SIGNAL PROTEIN | ||||||
Function / homology | ![]() glutathione transferase / glutathione transferase activity / glutathione metabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Tang, L. / Guo, B. / Van Wijnen, A.J. / Lian, J.B. / Stein, J.L. / Stein, G.S. / Zhou, G.W. | ||||||
![]() | ![]() Title: Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML-1/CBF-alpha2. Authors: Tang, L. / Guo, B. / van Wijnen, A.J. / Lian, J.B. / Stein, J.L. / Stein, G.S. / Zhou, G.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62.5 KB | Display | ![]() |
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PDB format | ![]() | 45.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 367.3 KB | Display | ![]() |
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Full document | ![]() | 388 KB | Display | |
Data in XML | ![]() | 8.7 KB | Display | |
Data in CIF | ![]() | 12.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gneS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32124.119 Da / Num. of mol.: 1 / Fragment: NMTS FRAGMENT Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 50 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: drop consists of equal volume of protein and reservoir solutions | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 85 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→15 Å / Num. obs: 7449 / % possible obs: 94.9 % / Observed criterion σ(I): 2 / Redundancy: 2.5 % / Biso Wilson estimate: 41 Å2 / Rsym value: 11 / Net I/σ(I): 3 |
Reflection shell | Resolution: 2.7→2.82 Å / Redundancy: 2 % / Mean I/σ(I) obs: 2.5 / Rsym value: 25 / % possible all: 87.5 |
Reflection | *PLUS Rmerge(I) obs: 0.11 |
Reflection shell | *PLUS % possible obs: 87.5 % / Rmerge(I) obs: 0.25 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1GNE Resolution: 2.7→6 Å / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.7→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.81 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.209 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor obs: 0.27 |