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- PDB-1mi4: Glyphosate insensitive G96A mutant EPSP synthase liganded with sh... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mi4 | ||||||
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Title | Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate | ||||||
![]() | 5-enolpyruvylshikimate-3-phosphate synthase | ||||||
![]() | TRANSFERASE / inside-out alpha-beta barrel | ||||||
Function / homology | ![]() 3-phosphoshikimate 1-carboxyvinyltransferase / 3-phosphoshikimate 1-carboxyvinyltransferase activity / shikimate kinase activity / shikimate 3-dehydrogenase (NADP+) activity / 3-dehydroquinate dehydratase activity / chorismate biosynthetic process / aromatic amino acid family biosynthetic process / amino acid biosynthetic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Eschenburg, S. / Healy, M.L. / Priestman, M.A. / Lushington, G.H. / Schonbrunn, E. | ||||||
![]() | ![]() Title: How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. Authors: Eschenburg, S. / Healy, M.L. / Priestman, M.A. / Lushington, G.H. / Schonbrunn, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106.5 KB | Display | ![]() |
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PDB format | ![]() | 79.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 799.1 KB | Display | ![]() |
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Full document | ![]() | 799.8 KB | Display | |
Data in XML | ![]() | 21.7 KB | Display | |
Data in CIF | ![]() | 34.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1g6tS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46155.637 Da / Num. of mol.: 1 / Mutation: G96A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P0A6D3, 3-phosphoshikimate 1-carboxyvinyltransferase | ||
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#2: Chemical | ChemComp-S3P / | ||
#3: Chemical | ChemComp-FMT / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.36 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 19 ℃ / pH: 7.8 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 10, 2001 / Details: confocal mirrors |
Radiation | Monochromator: confocal mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→15 Å / Num. all: 47038 / Num. obs: 47038 / % possible obs: 97.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Rmerge(I) obs: 0.054 |
Reflection shell | Resolution: 1.7→1.75 Å / Rmerge(I) obs: 0.346 / Num. unique all: 3815 / % possible all: 96 |
Reflection | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 15 Å / Num. measured all: 199475 |
Reflection shell | *PLUS Highest resolution: 1.7 Å / % possible obs: 96 % / Num. unique obs: 3815 / Num. measured obs: 16125 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1G6T Resolution: 1.7→15 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.7→15 Å
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Refine LS restraints |
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Xplor file |
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Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 15 Å / % reflection Rfree: 2.5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |