+Open data
-Basic information
Entry | Database: PDB / ID: 1mb1 | ||||||
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Title | MBP1 FROM SACCHAROMYCES CEREVISIAE | ||||||
Components | MLU1-BOX BINDING PROTEIN | ||||||
Keywords | TRANSCRIPTION REGULATION / CELL-CYCLE / TRANSCRIPTION FACTOR | ||||||
Function / homology | Function and homology information SBF transcription complex / MBF transcription complex / G1/S transition of mitotic cell cycle / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.1 Å | ||||||
Authors | Taylor, I.A. / Smerdon, S.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: The X-ray structure of the DNA-binding domain from the Saccharomyces cerevisiae cell-cycle transcription factor Mbp1 at 2.1 A resolution. Authors: Taylor, I.A. / Treiber, M.K. / Olivi, L. / Smerdon, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1mb1.cif.gz | 33.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1mb1.ent.gz | 22.3 KB | Display | PDB format |
PDBx/mmJSON format | 1mb1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1mb1_validation.pdf.gz | 416.1 KB | Display | wwPDB validaton report |
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Full document | 1mb1_full_validation.pdf.gz | 420.6 KB | Display | |
Data in XML | 1mb1_validation.xml.gz | 7.5 KB | Display | |
Data in CIF | 1mb1_validation.cif.gz | 9.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mb/1mb1 ftp://data.pdbj.org/pub/pdb/validation_reports/mb/1mb1 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14900.885 Da / Num. of mol.: 1 / Fragment: DNA-BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Cell line: BL21 / Cellular location: NUCLEAR / Plasmid: PET22B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P39678 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.88 Å3/Da / Density % sol: 28 % | ||||||||||||||||||||||||
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Crystal grow | pH: 6.9 / Details: pH 6.9 | ||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging dropDetails: Taylor, I.A., (1997) Proteins Struct. Funct. Genet., 27,325. PH range low: 8.2 / PH range high: 7.7 | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ELETTRA / Beamline: 5.2R / Wavelength: 1.22 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE AREA DETECTOR / Date: Jan 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.22 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→18 Å / Num. obs: 6810 / % possible obs: 94.4 % / Redundancy: 4.7 % / Biso Wilson estimate: 32 Å2 / Rmerge(I) obs: 0.041 / Net I/σ(I): 30 |
Reflection shell | Resolution: 2.1→2.16 Å / Rmerge(I) obs: 0.124 / Mean I/σ(I) obs: 11 / % possible all: 93.3 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.1→7 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso mean: 27 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→7 Å
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Refine LS restraints |
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Software | *PLUS Name: CCP4 / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.215 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_angle_deg / Dev ideal: 2.47 |