Back calculated data agree with experimental NOESY spectrum,structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy,target function
Representative
Model #21
closest to the average, minimized average structure
-
Components
#1: Protein
ChimeraofHumanandE. colithioredoxin
Mass: 11693.518 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Chimera consists of residues 1-66 from human, residues 67-107 from E. coli. Source: (gene. exp.) Homo sapiens, Escherichia coli / Plasmid: pET 11a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / References: UniProt: P10599, UniProt: P00274
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
HNCO
1
2
1
HN(CA)CB
1
3
1
15N TOCSY-HSQC
1
4
1
(H)CCH-TOCSY
1
5
1
H(CCO)NH-TOCSY
1
6
1
C(CO)NH-TOCSY
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Sample preparation
Details
Contents: 1 mM protein in 100 mM sodium phosphate buffer (pH 7.0), 20 M EDTA, 0.02% sodium azide, and 10% D2O. Solvent system: 90% H2O/10% D2O
Sample conditions
pH: 7 / Temperature: 308 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
500
1
Bruker DMX
Bruker
DMX
750
2
Bruker DMX
Bruker
DMX
600
3
Bruker DRX
Bruker
DRX
800
4
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Processing
NMR software
Name
Version
Classification
DYANA
1.5
structuresolution
XPLOR
refinement
Refinement
Method: see publication / Software ordinal: 1
NMR representative
Selection criteria: closest to the average, minimized average structure
NMR ensemble
Conformer selection criteria: Back calculated data agree with experimental NOESY spectrum,structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the ...Conformer selection criteria: Back calculated data agree with experimental NOESY spectrum,structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy,target function Conformers calculated total number: 100 / Conformers submitted total number: 21
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