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- PDB-1m0u: Crystal Structure of the Drosophila Glutathione S-transferase-2 i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1m0u | ||||||
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Title | Crystal Structure of the Drosophila Glutathione S-transferase-2 in Complex with Glutathione | ||||||
![]() | GST2 gene product | ||||||
![]() | TRANSFERASE / GST / Flight Muscle Protein / Sigma | ||||||
Function / homology | ![]() Glutathione conjugation / Synthesis of Prostaglandins (PG) and Thromboxanes (TX) / prostaglandin-D synthase activity / glutathione peroxidase activity / glutathione transferase / glutathione transferase activity / glutathione metabolic process / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Agianian, B. / Tucker, P.A. / Schouten, A. / Leonard, K. / Bullard, B. / Gros, P. | ||||||
![]() | ![]() Title: Structure of a Drosophila Sigma Class Glutathione S-transferase Reveals a Novel Active Site Topography Suited for Lipid Peroxidation Products Authors: Agianian, B. / Tucker, P.A. / Schouten, A. / Leonard, K. / Bullard, B. / Gros, P. #1: ![]() Title: Catalytic function of Drosophila melanogaster glutathione S-transferase DmGSTS1-1 (GST-2) in conjuction of lipid peroxidation end products Authors: Singh, S.P. / Coronella, J.A. / Benes, H. / Cochrane, B.J. / Zimniak, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.5 KB | Display | ![]() |
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PDB format | ![]() | 77.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 739.3 KB | Display | ![]() |
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Full document | ![]() | 744.3 KB | Display | |
Data in XML | ![]() | 20.5 KB | Display | |
Data in CIF | ![]() | 29.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The asymmetric unit contains the biological dimer |
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Components
#1: Protein | Mass: 27640.432 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-GSH / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.3 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Ammonium sulfate, Sodium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Details: Agianian, B., (2001) Acta Crystallogr., D57, 725. | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 16, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→76.7 Å / Num. all: 62202 / Num. obs: 62202 / % possible obs: 99.5 % / Rsym value: 0.039 |
Reflection shell | Resolution: 1.75→1.78 Å / Rsym value: 0.238 / % possible all: 99.9 |
Reflection | *PLUS Num. measured all: 623131 / Rmerge(I) obs: 0.039 |
Reflection shell | *PLUS % possible obs: 99.9 % / Rmerge(I) obs: 0.238 / Mean I/σ(I) obs: 9.14 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.5 Å2
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Refinement step | Cycle: LAST / Resolution: 1.75→70 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.795 Å / Total num. of bins used: 20 /
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Refinement | *PLUS Lowest resolution: 70 Å / Rfactor Rfree: 0.232 / Rfactor Rwork: 0.213 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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