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- PDB-1lup: Solution structure of a toxin (GsMTx2) from the tarantula, Grammo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lup | ||||||
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Title | Solution structure of a toxin (GsMTx2) from the tarantula, Grammostola spatulata, which inhibits mechanosensitive ion channels | ||||||
![]() | GsMTx2 | ||||||
![]() | TOXIN / inhibitor cysteine knot / beta-sheet | ||||||
Function / homology | toxin activity / extracellular region / Kappa-theraphotoxin-Gr2a![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
![]() | Oswald, R.E. / Suchyna, T.M. / McFeeters, R. / Gottlieb, P. / Sachs, F. | ||||||
![]() | ![]() Title: Solution structure of peptide toxins that block mechanosensitive ion channels Authors: Oswald, R.E. / Suchyna, T.M. / McFeeters, R. / Gottlieb, P. / Sachs, F. #1: ![]() Title: Identification of a peptide toxin from Grammostola spatulata spider venom that blocks stretch-activated channels. Authors: Suchyna, T.M. / Johnson, J.H. / Hamer, K. / Leykam, J.F. / Gage, D.A. / Clemo, H.F. / Baumgarten, C.M. / Sachs, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 207.5 KB | Display | ![]() |
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PDB format | ![]() | 178.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 339.4 KB | Display | ![]() |
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Full document | ![]() | 481.8 KB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 21.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3934.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() Secretion: venom / References: UniProt: P60273*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: H-D exchange using 2D TOCSY following resuspension of lyophilized protein in 100% D2O |
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Sample preparation
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |