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- PDB-1ln2: Crystal Structure of Human Phosphatidylcholine Transfer Protein i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ln2 | ||||||
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Title | Crystal Structure of Human Phosphatidylcholine Transfer Protein in Complex with Dilinoleoylphosphatidylcholine (Seleno-Met Protein) | ||||||
![]() | Phosphatidylcholine transfer protein | ||||||
![]() | LIPID BINDING PROTEIN / START Domain | ||||||
Function / homology | ![]() phosphatidylcholine transporter activity / Mitochondrial Fatty Acid Beta-Oxidation / phosphatidylcholine binding / phospholipid transport / negative regulation of cold-induced thermogenesis / lipid transport / Synthesis of PC / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Roderick, S.L. / Chan, W.W. / Agate, D.S. / Olsen, L.R. / Vetting, M.W. / Rajashankar, K.R. / Cohen, D.E. | ||||||
![]() | ![]() Title: Structure of human phosphatidylcholine transfer protein in complex with its ligand. Authors: Roderick, S.L. / Chan, W.W. / Agate, D.S. / Olsen, L.R. / Vetting, M.W. / Rajashankar, K.R. / Cohen, D.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95 KB | Display | ![]() |
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PDB format | ![]() | 79.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 547.1 KB | Display | ![]() |
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Full document | ![]() | 578.7 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 21.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a monomer. Two monomers are related by approximately (1/2,1/2,1/2). |
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Components
#1: Protein | Mass: 25199.564 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.72 Å3/Da / Density % sol: 66.94 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.7 Details: sodium formate, sodium acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 117 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 20, 2001 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→67.4 Å / Num. all: 16893 / Num. obs: 16893 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 28 Å2 / Rmerge(I) obs: 0.048 |
Reflection shell | Resolution: 2.9→3.08 Å / Rmerge(I) obs: 0.048 / % possible all: 93.5 |
Reflection | *PLUS Num. obs: 32130 / % possible obs: 99.7 % / Num. measured all: 164647 / Rmerge(I) obs: 0.048 |
Reflection shell | *PLUS Lowest resolution: 3 Å / % possible obs: 99.9 % / Rmerge(I) obs: 0.128 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 41.9758 Å2 / ksol: 0.383111 e/Å3 | ||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error free: 0.43 Å / Luzzati sigma a free: 0.71 Å | ||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→67.35 Å
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Refine LS restraints |
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LS refinement shell | Highest resolution: 2.9 Å / Total num. of bins used: 6 /
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Xplor file |
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor obs: 0.234 / Rfactor Rfree: 0.293 / Rfactor Rwork: 0.234 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 3 Å / Rfactor Rfree: 0.454 / Rfactor Rwork: 0.333 |