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- PDB-1lem: THE MONOSACCHARIDE BINDING SITE OF LENTIL LECTIN: AN X-RAY AND MO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lem | ||||||
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Title | THE MONOSACCHARIDE BINDING SITE OF LENTIL LECTIN: AN X-RAY AND MOLECULAR MODELLING STUDY | ||||||
![]() | (LECTIN) x 2 | ||||||
![]() | LECTIN | ||||||
Function / homology | ![]() carbohydrate mediated signaling / D-mannose binding / manganese ion binding / carbohydrate binding / calcium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Loris, R. / Wyns, L. | ||||||
![]() | ![]() Title: The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study. Authors: Loris, R. / Casset, F. / Bouckaert, J. / Pletinckx, J. / Dao-Thi, M.H. / Poortmans, F. / Imberty, A. / Perez, S. / Wyns, L. #1: ![]() Title: Crystal Structure Determination and Refinement at 2.3 Angstroms Resolution of the Lentil Lectin Authors: Loris, R. / Steyaert, J. / Maes, D. / Lisgarten, J. / Pickersgill, R. / Wyns, L. #2: ![]() Title: Two Crystal Forms of the Lentil Lectin Diffract to High Resolution Authors: Loris, R. / Lisgarten, J. / Maes, D. / Pickersgill, R. / Korber, F. / Reynolds, C. / Wyns, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.3 KB | Display | ![]() |
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PDB format | ![]() | 42.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 393.9 KB | Display | ![]() |
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Full document | ![]() | 405.4 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 11 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Atom site foot note | 1: ALA A 80 - ASP A 81 OMEGA = 0.73 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 19906.982 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Protein | Mass: 5714.288 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
-Sugars , 1 types, 1 molecules ![](data/chem/img/GLC.gif)
#3: Sugar | ChemComp-GLC / |
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-Non-polymers , 3 types, 45 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-CA / |
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#5: Chemical | ChemComp-MN / |
#6: Water | ChemComp-HOH / |
-Details
Nonpolymer details | THE LENTIL LECTIN MONOMER HAS A BOUND CALCIUM AND MANGANESE ION. THE CALCIUM AND MANGANESE IONS ARE ...THE LENTIL LECTIN MONOMER HAS A BOUND CALCIUM AND MANGANESE ION. THE CALCIUM AND MANGANESE IONS ARE ESSENTIAL FOR STABILIZIN |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 64.04 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 10 Å / Num. obs: 6950 / % possible obs: 94.5 % / Num. measured all: 35751 / Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Rfactor Rwork: 0.206 / Rfactor obs: 0.206 / Highest resolution: 3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 3 Å
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Refine LS restraints |
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Software | *PLUS Name: 'X-PLOR' / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 10 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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