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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1lcf | ||||||
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タイトル | CRYSTAL STRUCTURE OF COPPER-AND OXALATE-SUBSTITUTED HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION | ||||||
![]() | LACTOFERRIN | ||||||
![]() | IRON TRANSPORT | ||||||
機能・相同性 | ![]() negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism ...negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism / positive regulation of bone mineralization involved in bone maturation / negative regulation of lipopolysaccharide-mediated signaling pathway / specific granule / negative regulation of osteoclast development / antifungal humoral response / positive regulation of chondrocyte proliferation / negative regulation of ATP-dependent activity / regulation of tumor necrosis factor production / bone morphogenesis / Antimicrobial peptides / cysteine-type endopeptidase inhibitor activity / negative regulation of viral genome replication / positive regulation of osteoblast proliferation / humoral immune response / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; セリンエンドペプチターゼ / positive regulation of osteoblast differentiation / regulation of cytokine production / protein serine/threonine kinase activator activity / ossification / innate immune response in mucosa / secretory granule / lipopolysaccharide binding / positive regulation of protein serine/threonine kinase activity / recycling endosome / specific granule lumen / antimicrobial humoral immune response mediated by antimicrobial peptide / tertiary granule lumen / positive regulation of NF-kappaB transcription factor activity / heparin binding / iron ion transport / antibacterial humoral response / positive regulation of canonical NF-kappaB signal transduction / defense response to Gram-negative bacterium / killing of cells of another organism / early endosome / iron ion binding / Amyloid fiber formation / serine-type endopeptidase activity / Neutrophil degranulation / negative regulation of apoptotic process / cell surface / protein-containing complex / proteolysis / DNA binding / extracellular space / extracellular exosome / extracellular region / nucleus / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() | ||||||
![]() | Smith, C.A. / Anderson, B.F. / Baker, H.M. / Baker, E.N. | ||||||
![]() | ![]() タイトル: Structure of copper- and oxalate-substituted human lactoferrin at 2.0 A resolution. 著者: Smith, C.A. / Anderson, B.F. / Baker, H.M. / Baker, E.N. #1: ![]() タイトル: Anion Binding by Human Lactoferrin: Results from Crystallographic and Physicochemical Studies 著者: Shongwe, M.S. / Smith, C.A. / Ainscough, E.W. / Baker, H.M. / Brodie, A.M. / Baker, E.N. #2: ![]() タイトル: Preliminary Crystallographic Studies of Copper(II)-and Oxalate-Substituted Human Lactoferrin 著者: Smith, C.A. / Baker, H.M. / Baker, E.N. #3: ![]() タイトル: Structure of Human Lactoferrin: Crystallographic Structure Analysis and Refinement at 2.8 Angstroms Resolution 著者: Anderson, B.F. / Baker, H.M. / Norris, G.E. / Rice, D.W. / Baker, E.N. | ||||||
履歴 |
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Remark 700 | SHEET STRAND 4 OF SHEET *BN1* ON *SHEET* RECORDS BELOW ALSO APPEARS IN SHEET *BN2*. SHEET BN2 OF ...SHEET STRAND 4 OF SHEET *BN1* ON *SHEET* RECORDS BELOW ALSO APPEARS IN SHEET *BN2*. SHEET BN2 OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. STRANDS 1, 2, 3, AND 4 OF BN2 AND BN3 ARE IDENTICAL. STRAND 4 OF SHEET *BC1* ON *SHEET* RECORDS BELOW ALSO APPEARS IN SHEET *BC2*. SHEET BC2 OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. STRANDS 1, 2, 3, AND 4 OF BC2 AND BC3 ARE IDENTICAL. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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PDBx/mmCIF形式 | ![]() | 155.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 120.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 469.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 553.9 KB | 表示 | |
XML形式データ | ![]() | 41.7 KB | 表示 | |
CIF形式データ | ![]() | 56.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: CIS PROLINE - PRO 71 / 2: CIS PROLINE - PRO 142 / 3: CIS PROLINE - PRO 628 |
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要素
-タンパク質 / 糖 , 2種, 2分子 A![](data/chem/img/NAG.gif)
![](data/chem/img/NAG.gif)
#1: タンパク質 | 分子量: 76221.227 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() |
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#2: 糖 | ChemComp-NAG / |
-非ポリマー , 4種, 329分子 ![](data/chem/img/CU.gif)
![](data/chem/img/CO3.gif)
![](data/chem/img/OXL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CO3.gif)
![](data/chem/img/OXL.gif)
![](data/chem/img/HOH.gif)
#3: 化合物 | #4: 化合物 | ChemComp-CO3 / | #5: 化合物 | ChemComp-OXL / | #6: 水 | ChemComp-HOH / | |
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-詳細
配列の詳細 | SEQUENCE ADVISORY NOTICE DIFFERENCE BETWEEN SWISS-PROT AND PDB SEQUENCE. SWISS-PROT ENTRY NAME: ...SEQUENCE ADVISORY NOTICE DIFFERENCE |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.79 Å3/Da / 溶媒含有率: 55.87 % | ||||||||||||||||||||
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結晶化 | *PLUS 手法: microdialysis / PH range low: 8 / PH range high: 7.8 | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS 最高解像度: 2 Å / Num. obs: 38143 / % possible obs: 75 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.077 / Biso Wilson estimate: 34 Å2 |
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解析
ソフトウェア | 名称: PROFFT / 分類: 精密化 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 解像度: 2→8 Å / σ(F): 1 /
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精密化ステップ | サイクル: LAST / 解像度: 2→8 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: PROFFT / 分類: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.193 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 41.2 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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