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Yorodumi- PDB-1lap: MOLECULAR STRUCTURE OF LEUCINE AMINOPEPTIDASE AT 2.7-ANGSTROMS RE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1lap | ||||||
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Title | MOLECULAR STRUCTURE OF LEUCINE AMINOPEPTIDASE AT 2.7-ANGSTROMS RESOLUTION | ||||||
Components | Cytosol aminopeptidase | ||||||
Keywords | HYDROLASE(ALPHA-AMINOACYLPEPTIDE) | ||||||
Function / homology | Function and homology information cysteinylglycine-S-conjugate dipeptidase / prolyl aminopeptidase / leucyl aminopeptidase / dipeptidase activity / metalloaminopeptidase activity / carboxypeptidase activity / disordered domain specific binding / peptidase activity / manganese ion binding / mitochondrion ...cysteinylglycine-S-conjugate dipeptidase / prolyl aminopeptidase / leucyl aminopeptidase / dipeptidase activity / metalloaminopeptidase activity / carboxypeptidase activity / disordered domain specific binding / peptidase activity / manganese ion binding / mitochondrion / proteolysis / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.7 Å | ||||||
Authors | Burley, S.K. / David, P.R. / Taylor, A. / Lipscomb, W.N. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1990 Title: Molecular structure of leucine aminopeptidase at 2.7-A resolution. Authors: Burley, S.K. / David, P.R. / Taylor, A. / Lipscomb, W.N. #1: Journal: J.Mol.Biol. / Year: 1977 Title: Preliminary X-Ray Study of Leucine Aminopeptidase (Bovine Lens), an Oligomeric Metalloenzyme Authors: Jurnak, F. / Rich, A. / Vanloon-Klaassen, L. / Bloemendal, H. / Taylor, A. / Carpenter, F.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lap.cif.gz | 119.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lap.ent.gz | 93.4 KB | Display | PDB format |
PDBx/mmJSON format | 1lap.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1lap_validation.pdf.gz | 423.9 KB | Display | wwPDB validaton report |
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Full document | 1lap_full_validation.pdf.gz | 443.3 KB | Display | |
Data in XML | 1lap_validation.xml.gz | 20.1 KB | Display | |
Data in CIF | 1lap_validation.cif.gz | 26.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/la/1lap ftp://data.pdbj.org/pub/pdb/validation_reports/la/1lap | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 471 IS A CIS PROLINE. |
-Components
#1: Protein | Mass: 52942.098 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / References: UniProt: P00727, leucyl aminopeptidase | ||
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#2: Chemical | Sequence details | AT THE TIME OF DEPOSITION THE SEQUENCE PRESENTED IN PIR ENTRY APBOL DIFFERS FROM THE SEQUENCE ...AT THE TIME OF DEPOSITION | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.91 Å3/Da / Density % sol: 57.76 % |
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Conc.: 1-2 M / Chemical formula: Li2SO4 |
-Data collection
Reflection | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 20 Å / Num. obs: 16334 / Num. measured all: 275554 / Rmerge(I) obs: 0.102 |
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-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor Rwork: 0.169 / Highest resolution: 2.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.7 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.7 Å / Rfactor obs: 0.169 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d |