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Open data
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Basic information
Entry | Database: PDB / ID: 1la4 | ||||||
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Title | Solution Structure of SGTx1 | ||||||
![]() | SGTx1 | ||||||
![]() | TOXIN / Triple-stranded antiparallel beta-sheet / inhibitory cystine knot / Peptide neurotoxin | ||||||
Function / homology | Huwentoxin-1 family / Ion channel inhibitory toxin / ion channel inhibitor activity / : / toxin activity / extracellular space / Kappa-theraphotoxin-Scg1a![]() | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
![]() | Lee, C.W. / Roh, S.H. / Kim, S. / Endoh, H. / Kodera, Y. / Maeda, T. / Swartz, K.J. / Kim, J.I. | ||||||
![]() | ![]() Title: Solution Structure and Functional Characterization of SGTx1, a Modifier of Kv2.1 Channel Gating Authors: Lee, C.W. / Kim, S. / Roh, S.H. / Endoh, H. / Kodera, Y. / Maeda, T. / Kohno, T. / Wang, J.M. / Swartz, K.J. / Kim, J.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 194.7 KB | Display | ![]() |
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PDB format | ![]() | 168.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 339.7 KB | Display | ![]() |
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Full document | ![]() | 470.7 KB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 28.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3788.364 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: This sequence is chemically synthesized. / References: UniProt: P56855 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
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Sample preparation
Details | Contents: 2mM SGTx1; 90% H2O, 10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 0 / pH: 3.5 / Pressure: ambient / Temperature: 310 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 Details: Additional comments about the NMR refinement can be placed here, e.g. the structures are based on a total of 499 restraints, 449 are NOE-derived distance constraints, 27 dihedral angle ...Details: Additional comments about the NMR refinement can be placed here, e.g. the structures are based on a total of 499 restraints, 449 are NOE-derived distance constraints, 27 dihedral angle restraints,14 distance restraints from hydrogen bonds, and 9 distance restraints from disulfide bonds. | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average,lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |