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- PDB-1l3o: SOLUTION STRUCTURE DETERMINATION OF THE FULLY OXIDIZED DOUBLE MUT... -
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Basic information
Entry | Database: PDB / ID: 1l3o | ||||||
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Title | SOLUTION STRUCTURE DETERMINATION OF THE FULLY OXIDIZED DOUBLE MUTANT K9-10A CYTOCHROME C7 FROM DESULFUROMONAS ACETOXIDANS, ENSEMBLE OF 35 STRUCTURES | ||||||
![]() | cytochrome c7 | ||||||
![]() | OXYGEN STORAGE/TRANSPORT / automatic assignment / cytochrome c7 / electron transfer / multiheme cytochromes / NMR solution structures / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | ![]() anaerobic respiration / electron transfer activity / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing in torsion angle space, restrained energy minimization | ||||||
![]() | Assfalg, M. / Bertini, I. / Turano, P. / Bruschi, M. / Durand, M.C. / Giudici-Orticoni, M.T. / Dolla, A. | ||||||
![]() | ![]() Title: A quick solution structure determination of the fully oxidized double mutant K9-10A cytochrome c7 from Desulfuromonas acetoxidans and mechanistic implications. Authors: Assfalg, M. / Bertini, I. / Turano, P. / Bruschi, M. / Durand, M.C. / Giudici-Orticoni, M.T. / Dolla, A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 810.5 KB | Display | ![]() |
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PDB format | ![]() | 704.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 592.9 KB | Display | ![]() |
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Full document | ![]() | 804.3 KB | Display | |
Data in XML | ![]() | 45 KB | Display | |
Data in CIF | ![]() | 73.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1kwjC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 7164.105 Da / Num. of mol.: 1 / Mutation: K9A, K10A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY |
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Sample preparation
Details | Contents: 2mM cytochrome c7 Solvent system: 100mM phosphate buffer; pH 6.5; 90% H2O, 10% D2O |
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Sample conditions | Ionic strength: 100 mM phosphate buffer / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing in torsion angle space, restrained energy minimization Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 35 structures with the lowest target function values Conformers calculated total number: 500 / Conformers submitted total number: 35 |