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Yorodumi- PDB-1kuq: CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1kuq | ||||||
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Title | CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA | ||||||
Components |
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Keywords | RIBOSOME / rRNA-protein complex | ||||||
Function / homology | Function and homology information rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.84 Å | ||||||
Authors | Nikulin, A.D. / Tishchenko, S. / Revtovich, S. / Ehresmann, B. / Ehresmann, C. / Dumas, P. / Garber, M. / Nikonov, S. / Nevskaya, N. | ||||||
Citation | Journal: Biochemistry Mosc. / Year: 2004 Title: Role of N-terminal helix in interaction of ribosomal protein S15 with 16S rRNA. Authors: Revtovich, S.V. / Nikulin, A.D. / Nikonov, S.V. #1: Journal: Nat.Struct.Biol. / Year: 2000 Title: Crystal structure of the S15-rRNA complex Authors: Nikulin, A. / Serganov, A. / Ennifar, E. / Tishchenko, S. / Nevskaya, N. / Shepard, W. / Portier, C. / Garber, M. / Ehresmann, B. / Ehresmann, C. / Nikonov, S. / Dumas, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kuq.cif.gz | 65.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kuq.ent.gz | 45.3 KB | Display | PDB format |
PDBx/mmJSON format | 1kuq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ku/1kuq ftp://data.pdbj.org/pub/pdb/validation_reports/ku/1kuq | HTTPS FTP |
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-Related structure data
Related structure data | 1dk1S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 18464.963 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Production host: Escherichia coli (E. coli) / Strain (production host): DH1 |
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#2: Protein | Mass: 10366.200 Da / Num. of mol.: 1 / Mutation: T3C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Production host: Escherichia coli (E. coli) / Strain (production host): B834 / References: UniProt: P80378 |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.98 % | ||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: ammonium sulphate, KCl, MgCl2, sodium cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.933 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 21, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.933 Å / Relative weight: 1 |
Reflection | Resolution: 2.84→25 Å / Num. all: 7828 / Num. obs: 7828 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.6 % / Biso Wilson estimate: 117.9 Å2 / Rmerge(I) obs: 0.049 |
Reflection shell | Resolution: 2.84→2.92 Å / Rmerge(I) obs: 0.12 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1DK1 Resolution: 2.84→24.41 Å / Rfactor Rfree error: 0.016 / Data cutoff high absF: 1157536.51 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 69.8947 Å2 / ksol: 0.362129 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.84→24.41 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.84→3.02 Å / Rfactor Rfree error: 0.06 / Total num. of bins used: 6
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Xplor file |
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