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Yorodumi- PDB-1dk1: DETAILED VIEW OF A KEY ELEMENT OF THE RIBOSOME ASSEMBLY: CRYSTAL ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dk1 | ||||||
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Title | DETAILED VIEW OF A KEY ELEMENT OF THE RIBOSOME ASSEMBLY: CRYSTAL STRUCTURE OF THE S15-RRNA COMPLEX | ||||||
Components |
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Keywords | RIBOSOME / S15 / PROTEIN / RNA | ||||||
Function / homology | Function and homology information rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.8 Å | ||||||
Authors | Nikulin, A. / Serganov, A. / Ennifar, E. / Tischenko, S. / Nevskaya, N. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Crystal structure of the S15-rRNA complex. Authors: Nikulin, A. / Serganov, A. / Ennifar, E. / Tishchenko, S. / Nevskaya, N. / Shepard, W. / Portier, C. / Garber, M. / Ehresmann, B. / Ehresmann, C. / Nikonov, S. / Dumas, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dk1.cif.gz | 61.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dk1.ent.gz | 47.4 KB | Display | PDB format |
PDBx/mmJSON format | 1dk1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dk/1dk1 ftp://data.pdbj.org/pub/pdb/validation_reports/dk/1dk1 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-RNA chain / Protein , 2 types, 2 molecules BA
#1: RNA chain | Mass: 18464.963 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Production host: Escherichia coli (E. coli) |
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#2: Protein | Mass: 10498.702 Da / Num. of mol.: 1 / Fragment: RESIDUES 2-87 / Mutation: ILE11MSE, ALA79MSE Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P80378 |
-Non-polymers , 4 types, 37 molecules
#3: Chemical | ChemComp-MG / #4: Chemical | #5: Chemical | ChemComp-K / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.29 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: 100MM SODIUM CACODYLATE, 30 MM KCL, 1M (NH4)2SO4, 1.5 MM MGCL2, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 22K | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion / Details: drop:reservoir in a 3:2 volume ratio | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7A / Wavelength: 0.94654 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 18, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.94654 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. obs: 8164 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 8.1 % / Rmerge(I) obs: 0.038 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 8.3 % / Rmerge(I) obs: 0.209 / % possible all: 99.5 |
Reflection shell | *PLUS % possible obs: 99.5 % |
-Processing
Software |
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Refinement | Resolution: 2.8→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 2 / % reflection Rfree: 8.3 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.4 |