+Open data
-Basic information
Entry | Database: PDB / ID: 1kq8 | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of Winged Helix Protein HFH-1 | ||||||
Components | HEPATOCYTE NUCLEAR FACTOR 3 FORKHEAD HOMOLOG 1 | ||||||
Keywords | TRANSCRIPTION / Winged Helix Protein / HFH-1 | ||||||
Function / homology | Function and homology information hair follicle morphogenesis / anatomical structure morphogenesis / DNA-binding transcription repressor activity, RNA polymerase II-specific / negative regulation of neuron apoptotic process / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Sheng, W. / Rance, M. / Liao, X. | ||||||
Citation | Journal: Biochemistry / Year: 2002 Title: Structure comparison of two conserved HNF-3/fkh proteins HFH-1 and genesis indicates the existence of folding differences in their complexes with a DNA binding sequence. Authors: Sheng, W. / Rance, M. / Liao, X. #1: Journal: J.Mol.Biol. / Year: 1998 Title: Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins Authors: Marsden, I. / Jin, C. / Liao, X. #2: Journal: Biochemistry / Year: 1998 Title: Sequence specific collective motions in a winged helix DNA binding domain detected by 15N relaxation NMR Authors: Jin, C. / Marsden, I. / Chen, X. / Liao, X. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1kq8.cif.gz | 481.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1kq8.ent.gz | 401.4 KB | Display | PDB format |
PDBx/mmJSON format | 1kq8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1kq8_validation.pdf.gz | 342.2 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1kq8_full_validation.pdf.gz | 524.1 KB | Display | |
Data in XML | 1kq8_validation.xml.gz | 37.4 KB | Display | |
Data in CIF | 1kq8_validation.cif.gz | 55.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kq/1kq8 ftp://data.pdbj.org/pub/pdb/validation_reports/kq/1kq8 | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 11804.493 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Production host: Escherichia coli (E. coli) / References: UniProt: Q63244 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Sample conditions | pH: 6.5 / Temperature: 290 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software | Name: DYANA / Version: 1.5 / Developer: Gunter, P. / Classification: refinement |
---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 |
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 |