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- PDB-1kg9: Structure of a "mock-trapped" early-M intermediate of bacteriorho... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1kg9 | ||||||
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Title | Structure of a "mock-trapped" early-M intermediate of bacteriorhosopsin | ||||||
![]() | bacteriorhodopsin | ||||||
![]() | PROTON TRANSPORT / bacteriorhodopsin / M-intermediate / control | ||||||
Function / homology | ![]() light-driven active monoatomic ion transmembrane transporter activity / photoreceptor activity / phototransduction / proton transmembrane transport / monoatomic ion channel activity / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Facciotti, M.T. / Rouhani, S. / Burkard, F.T. / Betancourt, F.M. / Downing, K.H. / Rose, R.B. / McDermott, G. / Glaeser, R.M. | ||||||
![]() | ![]() Title: Structure of an early intermediate in the M-state phase of the bacteriorhodopsin photocycle. Authors: Facciotti, M.T. / Rouhani, S. / Burkard, F.T. / Betancourt, F.M. / Downing, K.H. / Rose, R.B. / McDermott, G. / Glaeser, R.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61.9 KB | Display | ![]() |
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PDB format | ![]() | 42.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1kg8C ![]() 1kgbC ![]() 1c3wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25301.916 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() | ||
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#2: Chemical | ChemComp-RET / | ||
#3: Chemical | ChemComp-LI1 / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.91 % | ||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: mono-olein cubic phase / pH: 5.6 Details: 3.0M Na/K Phosphate, pH 5.6, mono-olein cubic phase, temperature 293K | ||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 22, 2000 |
Radiation | Monochromator: NULL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. all: 20519 / Num. obs: 20519 / % possible obs: 97.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.05 / Net I/σ(I): 26.1 |
Reflection shell | Resolution: 1.81→1.9 Å / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 5.2 / % possible all: 99.7 |
Reflection | *PLUS Lowest resolution: 20 Å / Rmerge(I) obs: 0.05 |
Reflection shell | *PLUS % possible obs: 99.7 % / Rmerge(I) obs: 0.41 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1C3W Resolution: 1.81→20 Å / σ(F): 0 / Stereochemistry target values: NULL
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Refinement step | Cycle: LAST / Resolution: 1.81→20 Å
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Software | *PLUS Name: SHELXL / Version: 97 / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Num. reflection obs: 19440 / Rfactor all: 0.233 / Rfactor obs: 0.221 / Rfactor Rfree: 0.293 / Rfactor Rwork: 0.221 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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