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- PDB-1kfs: DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX -
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Open data
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Basic information
Entry | Database: PDB / ID: 1kfs | ||||||
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Title | DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX | ||||||
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![]() | TRANSFERASE/DNA / COMPLEX (HYDROLASE-DNA) / EXONUCLEASE / PHOSPHOROTHIOATE / TRANSFERASE-DNA COMPLEX | ||||||
Function / homology | ![]() 5'-3' exonuclease activity / double-strand break repair via alternative nonhomologous end joining / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair ...5'-3' exonuclease activity / double-strand break repair via alternative nonhomologous end joining / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair / DNA binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Brautigam, C.A. / Steitz, T.A. | ||||||
![]() | ![]() Title: Structural principles for the inhibition of the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I by phosphorothioates. Authors: Brautigam, C.A. / Steitz, T.A. #1: ![]() Title: Structural Basis for the 3'-5' Exonuclease Activity of Escherichia coli DNA Polymerase I: A Two Metal Ion Mechanism Authors: Beese, L.S. / Steitz, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 145.5 KB | Display | ![]() |
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PDB format | ![]() | 109.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.4 KB | Display | ![]() |
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Full document | ![]() | 458.8 KB | Display | |
Data in XML | ![]() | 28.3 KB | Display | |
Data in CIF | ![]() | 40.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 2113.410 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||
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#2: Protein | Mass: 68193.750 Da / Num. of mol.: 1 / Mutation: V324M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() | ||
#3: Chemical | ChemComp-MG / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.3 Å3/Da / Density % sol: 64 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.8 / Details: 1.4 M NA CITRATE PH 5.8 | ||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 64 % | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7 / Method: vapor diffusion, hanging drop / Details: Brick, P., (1983) J. Mol. Biol., 166, 453. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: SI CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 289824 / % possible obs: 89.1 % / Observed criterion σ(I): -3 / Rsym value: 5.5 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2.1→2.14 Å / Redundancy: 2 % / Mean I/σ(I) obs: 2.2 / Rsym value: 28.5 / % possible all: 94.4 |
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 20 Å / Num. obs: 77876 / % possible obs: 89.1 % / Num. measured all: 289824 / Rmerge(I) obs: 0.055 |
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Processing
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Refinement | Method to determine structure: RIGID-BODY REFINEMENT Starting model: IN-HOUSE HIGH-RESOLUTION STRUCTURE Resolution: 2.1→20 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / Cross valid method: THROUGHOUT / σ(F): 2 Details: SINCE DATA WERE COLLECTED AT A WAVELENGTH AT WHICH ZN++ SCATTERS ANOMALOUSLY, THE FOURIER MATES WERE TREATED SEPARATELY AND THE ANOMALOUS AND IMAGINARY FLAGS WERE USED. NO DENSITY COULD BE ...Details: SINCE DATA WERE COLLECTED AT A WAVELENGTH AT WHICH ZN++ SCATTERS ANOMALOUSLY, THE FOURIER MATES WERE TREATED SEPARATELY AND THE ANOMALOUS AND IMAGINARY FLAGS WERE USED. NO DENSITY COULD BE LOCATED FOR THE 5'-MOST FOUR BASES.
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Displacement parameters | Biso mean: 37.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å / Luzzati d res low obs: 20 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.12 Å / Total num. of bins used: 30
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 10 % / Rfactor obs: 0.195 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 37.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.3018 / Rfactor Rwork: 0.2645 |