[English] 日本語
![](img/lk-miru.gif)
- PDB-1kfd: CRYSTAL STRUCTURES OF THE KLENOW FRAGMENT OF DNA POLYMERASE I COM... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1kfd | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURES OF THE KLENOW FRAGMENT OF DNA POLYMERASE I COMPLEXED WITH DEOXYNUCLEOSIDE TRIPHOSPHATE AND PYROPHOSPHATE | ||||||
![]() | DNA POLYMERASE I KLENOW FRAGMENT | ||||||
![]() | NUCLEOTIDYLTRANSFERASE | ||||||
Function / homology | ![]() 5'-3' exonuclease activity / double-strand break repair via alternative nonhomologous end joining / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair ...5'-3' exonuclease activity / double-strand break repair via alternative nonhomologous end joining / 3'-5' exonuclease activity / base-excision repair / DNA-templated DNA replication / double-strand break repair / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair / DNA binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Beese, L.S. / Friedman, J.M. / Steitz, T.A. | ||||||
![]() | ![]() Title: Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate. Authors: Beese, L.S. / Friedman, J.M. / Steitz, T.A. #1: ![]() Title: Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA Authors: Beese, L.S. / Derbyshire, V. / Steitz, T.A. #2: ![]() Title: Structural Basis for the 3'-5' Exonuclease Activity of E. Coli DNA Polymerase F: A Two Metal Ion Mechanism Authors: Beese, L.S. / Steitz, T.A. #3: ![]() Title: Co-Crystal Structure of an Editing Complex of Klenow Fragment with DNA Authors: Freemont, P.S. / Friedman, J.M. / Beese, L.S. / Steitz, T.A. #4: ![]() Title: Structure of Large Fragment of Escherichia Coli DNA Polymerase I Complexed with dTMP Authors: Ollis, D.L. / Brick, P. / Hamlin, R. / Xuong, N.G. / Steitz, T.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 110.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 82 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 469.8 KB | Display | |
Data in XML | ![]() | 14.6 KB | Display | |
Data in CIF | ![]() | 21.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 68161.688 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
---|---|
#2: Chemical | ChemComp-CTP / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.43 Å3/Da / Density % sol: 64.14 % | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 6 / Method: vapor diffusion / Details: Brick, P., (1983) J.Mol.Biol., 166, 453. | |||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
-
Processing
Refinement | Highest resolution: 3.9 Å | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement step | Cycle: LAST / Highest resolution: 3.9 Å
|