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Yorodumi- PDB-1ka5: Refined Solution Structure of Histidine Containing Phosphocarrier... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ka5 | |||||||||
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Title | Refined Solution Structure of Histidine Containing Phosphocarrier Protein from Staphyloccocus aureus | |||||||||
Components | PHOSPHOCARRIER PROTEIN HPR | |||||||||
Keywords | LIGAND TRANSPORT / Open Faced beta-Sandwich / Structural Proteomics in Europe / SPINE / Structural Genomics | |||||||||
Function / homology | Function and homology information phosphoenolpyruvate-dependent sugar phosphotransferase system / cytoplasm Similarity search - Function | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Maurer, T. / Meier, S. / Hengstenberg, W. / Kalbitzer, H.R. / Structural Proteomics in Europe (SPINE) | |||||||||
Citation | Journal: J.Bacteriol. / Year: 2004 Title: High-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus and characterization of its interaction with the bifunctional HPr kinase/phosphorylase Authors: Maurer, T. / Meier, S. / Kachel, N. / Munte, C.E. / Hasenbein, S. / Koch, B. / Hengstenberg, W. / Kalbitzer, H.R. #1: Journal: Eur.J.Biochem. / Year: 1993 Title: The Solution Structure of the Histidine Containing Phosphocarrier Protein (HPr) from Staphylococcus aureus as Determined by Two-Dimensional 1H-NMR Spectroscopy Authors: Kalbitzer, H.R. / Hengstenberg, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ka5.cif.gz | 416.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ka5.ent.gz | 346.5 KB | Display | PDB format |
PDBx/mmJSON format | 1ka5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ka5_validation.pdf.gz | 342.9 KB | Display | wwPDB validaton report |
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Full document | 1ka5_full_validation.pdf.gz | 466.9 KB | Display | |
Data in XML | 1ka5_validation.xml.gz | 54.2 KB | Display | |
Data in CIF | 1ka5_validation.cif.gz | 68.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ka/1ka5 ftp://data.pdbj.org/pub/pdb/validation_reports/ka/1ka5 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9504.689 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Staphylococcus aureus (bacteria) / References: UniProt: P0A0E3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2mM HPr / Solvent system: 95% H2O, 5% 2H2O |
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Sample conditions | Ionic strength: 0 / pH: 7.4 / Pressure: ambinet / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: 1883 distance and angle restraints and hydrogen bonding patterns | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 440 / Conformers submitted total number: 16 |