+Open data
-Basic information
Entry | Database: PDB / ID: 1k8r | ||||||
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Title | Crystal structure of Ras-Bry2RBD complex | ||||||
Components |
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Keywords | SIGNALING PROTEIN / signal transduction / cancer / GTPase / ubiquitin fold | ||||||
Function / homology | Function and homology information Oxidative Stress Induced Senescence / induction of conjugation with cellular fusion / pheromone response MAPK cascade / division septum / cell tip / mitogen-activated protein kinase kinase kinase / GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity ...Oxidative Stress Induced Senescence / induction of conjugation with cellular fusion / pheromone response MAPK cascade / division septum / cell tip / mitogen-activated protein kinase kinase kinase / GTPase complex / oncogene-induced cell senescence / positive regulation of ruffle assembly / negative regulation of GTPase activity / MAP kinase kinase kinase activity / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of miRNA metabolic process / T-helper 1 type immune response / positive regulation of wound healing / cell division site / defense response to protozoan / Signaling by RAS GAP mutants / Signaling by RAS GTPase mutants / Activation of RAS in B cells / RAS signaling downstream of NF1 loss-of-function variants / SOS-mediated signalling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / SHC1 events in ERBB4 signaling / GRB2 events in EGFR signaling / EGFR Transactivation by Gastrin / SHC1 events in EGFR signaling / positive regulation of protein targeting to membrane / Signalling to RAS / GRB2 events in ERBB2 signaling / SHC-related events triggered by IGF1R / Activated NTRK2 signals through FRS2 and FRS3 / Estrogen-stimulated signaling through PRKCZ / adipose tissue development / SHC1 events in ERBB2 signaling / SHC-mediated cascade:FGFR3 / MET activates RAS signaling / : / Signaling by PDGFRA transmembrane, juxtamembrane and kinase domain mutants / Signaling by PDGFRA extracellular domain mutants / Schwann cell development / SHC-mediated cascade:FGFR2 / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / SHC-mediated cascade:FGFR4 / Signaling by FGFR4 in disease / Erythropoietin activates RAS / SHC-mediated cascade:FGFR1 / protein-membrane adaptor activity / FRS-mediated FGFR3 signaling / positive regulation of epithelial cell proliferation / Signaling by FLT3 ITD and TKD mutants / FRS-mediated FGFR2 signaling / Signaling by FGFR3 in disease / FRS-mediated FGFR4 signaling / p38MAPK events / Tie2 Signaling / FRS-mediated FGFR1 signaling / Signaling by FGFR2 in disease / EPHB-mediated forward signaling / Signaling by FLT3 fusion proteins / FLT3 Signaling / Ras activation upon Ca2+ influx through NMDA receptor / myelination / Signaling by FGFR1 in disease / CD209 (DC-SIGN) signaling / NCAM signaling for neurite out-growth / Downstream signal transduction / Constitutive Signaling by Overexpressed ERBB2 / Insulin receptor signalling cascade / intrinsic apoptotic signaling pathway / Signaling by ERBB2 TMD/JMD mutants / small monomeric GTPase / positive regulation of MAP kinase activity / Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants / G protein activity / positive regulation of GTPase activity / VEGFR2 mediated cell proliferation / Constitutive Signaling by EGFRvIII / Signaling by ERBB2 ECD mutants / Signaling by ERBB2 KD Mutants / regulation of actin cytoskeleton organization / FCERI mediated MAPK activation / animal organ morphogenesis / positive regulation of JNK cascade / RAF activation / regulation of long-term neuronal synaptic plasticity / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / Signaling by SCF-KIT / cellular senescence / cellular response to gamma radiation / positive regulation of fibroblast proliferation / Regulation of RAS by GAPs / RAS processing / Negative regulation of MAPK pathway / endocytosis / positive regulation of type II interferon production / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Schizosaccharomyces pombe (fission yeast) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Scheffzek, K. / Gruenewald, P. / Wohlgemuth, S. / Kabsch, W. / Tu, H. / Wigler, M. / Wittinghofer, A. / Herrmann, C. | ||||||
Citation | Journal: Structure / Year: 2001 Title: The Ras-Byr2RBD complex: structural basis for Ras effector recognition in yeast. Authors: Scheffzek, K. / Grunewald, P. / Wohlgemuth, S. / Kabsch, W. / Tu, H. / Wigler, M. / Wittinghofer, A. / Herrmann, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1k8r.cif.gz | 65.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1k8r.ent.gz | 47.3 KB | Display | PDB format |
PDBx/mmJSON format | 1k8r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1k8r_validation.pdf.gz | 756.3 KB | Display | wwPDB validaton report |
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Full document | 1k8r_full_validation.pdf.gz | 761.7 KB | Display | |
Data in XML | 1k8r_validation.xml.gz | 8.9 KB | Display | |
Data in CIF | 1k8r_validation.cif.gz | 11.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k8/1k8r ftp://data.pdbj.org/pub/pdb/validation_reports/k8/1k8r | HTTPS FTP |
-Related structure data
Related structure data | 5p21S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18875.191 Da / Num. of mol.: 1 / Fragment: GTP-binding/catalytic domain, residues 1-166 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: Hras or Hras1 / Plasmid: pGEX-2T / Production host: Escherichia coli (E. coli) / Strain (production host): CK600K / References: UniProt: P01112 |
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#2: Protein | Mass: 12426.270 Da / Num. of mol.: 1 / Fragment: Ras binding domain (RBD), residues 71-180 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schizosaccharomyces pombe (fission yeast) Gene: BYR2 or STE8 or SPBC1D7.05 / Plasmid: pGEX-2T / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 References: UniProt: P28829, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-GNP / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 4 |
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-Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 63.29 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ROTATING ANODE / Type: ELLIOTT GX-18 / Wavelength: 1.54 Å |
Detector | Type: SIEMENS-NICOLET / Detector: AREA DETECTOR / Date: Aug 6, 1996 / Details: mirrors |
Radiation | Monochromator: Ni Mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 3→25 Å / Num. all: 8592 / Num. obs: 8526 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 12.8 % / Biso Wilson estimate: 56 Å2 / Rmerge(I) obs: 0.139 / Rsym value: 0.139 / Net I/σ(I): 18.6 |
Reflection shell | Resolution: 3→3.1 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 4.1 / Num. unique all: 788 / Rsym value: 0.3 / % possible all: 99.9 |
Reflection | *PLUS Highest resolution: 3 Å / Lowest resolution: 25 Å / Num. measured all: 109981 |
Reflection shell | *PLUS Highest resolution: 3 Å / % possible obs: 100 % / Num. unique obs: 788 / Num. measured obs: 2634 / Rmerge(I) obs: 0.305 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 5p21 Resolution: 3→25 Å / Rfactor Rfree error details: 0.01 / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: residues 127-139 and 166-180 of Byr2 are ill defined in electron density and are not included in the model
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.19 Å / Rfactor Rfree error: 0.029 / Total num. of bins used: 6
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Refinement | *PLUS Highest resolution: 3 Å / Lowest resolution: 25 Å / σ(F): 0 / % reflection Rfree: 10 % / Rfactor obs: 0.236 / Rfactor Rfree: 0.312 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 3 Å / Rfactor Rfree: 0.35 / % reflection Rfree: 10.3 % / Rfactor Rwork: 0.297 |