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- PDB-1k82: Crystal structure of E.coli formamidopyrimidine-DNA glycosylase (... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1k82 | ||||||
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Title | Crystal structure of E.coli formamidopyrimidine-DNA glycosylase (Fpg) covalently trapped with DNA | ||||||
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![]() | HYDROLASE/DNA / PROTEIN-DNA COMPLEX / DNA REPAIR / BETA SANDWICH / ZINC FINGER / HELIX TWO-TURNS HELIX / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | ![]() oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity / oxidized purine nucleobase lesion DNA N-glycosylase activity / DNA-formamidopyrimidine glycosylase / base-excision repair, AP site formation / DNA N-glycosylase activity / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / nucleotide-excision repair ...oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity / oxidized purine nucleobase lesion DNA N-glycosylase activity / DNA-formamidopyrimidine glycosylase / base-excision repair, AP site formation / DNA N-glycosylase activity / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / nucleotide-excision repair / base-excision repair / endonuclease activity / damaged DNA binding / DNA damage response / zinc ion binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gilboa, R. / Zharkov, D.O. / Golan, G. / Fernandes, A.S. / Gerchman, S.E. / Matz, E. / Kycia, J.H. / Grollman, A.P. / Shoham, G. | ||||||
![]() | ![]() Title: Structure of formamidopyrimidine-DNA glycosylase covalently complexed to DNA. Authors: Gilboa, R. / Zharkov, D.O. / Golan, G. / Fernandes, A.S. / Gerchman, S.E. / Matz, E. / Kycia, J.H. / Grollman, A.P. / Shoham, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 290 KB | Display | ![]() |
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PDB format | ![]() | 217.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.3 KB | Display | ![]() |
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Full document | ![]() | 529.9 KB | Display | |
Data in XML | ![]() | 31.9 KB | Display | |
Data in CIF | ![]() | 50.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3934.546 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: DNA chain | Mass: 3863.531 Da / Num. of mol.: 4 / Source method: obtained synthetically #3: Protein | Mass: 30203.910 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P05523, DNA-formamidopyrimidine glycosylase #4: Chemical | ChemComp-ZN / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 49.04 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 8000, Ammonium Sulfate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 15 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 29, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→34 Å / Num. all: 84396 / Num. obs: 84396 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 33 Å2 / Rsym value: 0.069 / Net I/σ(I): 11.8 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 4 % / Num. unique all: 8405 / Rsym value: 0.265 / % possible all: 99.7 |
Reflection | *PLUS Lowest resolution: 34 Å / Num. measured all: 310926 / Rmerge(I) obs: 0.069 |
Reflection shell | *PLUS % possible obs: 99.7 % / Rmerge(I) obs: 0.265 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EE8 AND PDB ENTRY 1K3W Resolution: 2.1→34 Å / Isotropic thermal model: RESTRAINED / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Displacement parameters | Biso mean: 37 Å2 | ||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.27 Å / Num. disordered residues: 0 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→34 Å
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Refine LS restraints |
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rfree: 0.265 / Rfactor Rwork: 0.214 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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