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- PDB-1k81: NMR Structure of the Zinc-Ribbon Domain within Translation Initia... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1k81 | ||||||
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Title | NMR Structure of the Zinc-Ribbon Domain within Translation Initiation Factor 2 Subunit beta | ||||||
![]() | PROBABLE TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT | ||||||
![]() | TRANSLATION / translation initiation factor / zinc ribbon | ||||||
Function / homology | ![]() formation of cytoplasmic translation initiation complex / formation of translation preinitiation complex / translation initiation factor binding / translation initiation factor activity / mRNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Cho, S. / Hoffman, D.W. | ||||||
![]() | ![]() Title: Structure of the beta Subunit of Translation Initiation Factor 2 from the Archaeon Methanococcus jannaschii: A Representative of the eIF2beta/eIF5 Family of Proteins Authors: Cho, S. / Hoffman, D.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.2 KB | Display | ![]() |
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PDB format | ![]() | 98.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 337.2 KB | Display | ![]() |
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Full document | ![]() | 390.2 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 12.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 4062.103 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: pET14b / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D and 3D heteronuclear NMR techniques. |
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Sample preparation
Details | Contents: 1 mM aIF2 beta U-15N,13C; 10 mM phosphate buffer / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 50 mM / pH: 6.0 / Pressure: ambient / Temperature: 293 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
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Processing
NMR software | Name: CNS / Version: 1 / Developer: Brunger / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 170 / Conformers submitted total number: 10 |