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Open data
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Basic information
Entry | Database: PDB / ID: 1k3s | ||||||
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Title | Type III Secretion Chaperone SigE | ||||||
![]() | SigE | ||||||
![]() | CHAPERONE / Type III / secretion / SigE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bertero, M.G. / Luo, Y. / Frey, E.A. / Pfuetzner, R.A. / Wenk, M.R. / Creagh, L. / Marcus, S.L. / Lim, D. / Finlay, B.B. / Strynadka, N.C.J. | ||||||
![]() | ![]() Title: Structural and biochemical characterization of the type III secretion chaperones CesT and SigE. Authors: Luo, Y. / Bertero, M.G. / Frey, E.A. / Pfuetzner, R.A. / Wenk, M.R. / Creagh, L. / Marcus, S.L. / Lim, D. / Sicheri, F. / Kay, C. / Haynes, C. / Finlay, B.B. / Strynadka, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50.8 KB | Display | ![]() |
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PDB format | ![]() | 40.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a dimer |
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Components
#1: Protein | Mass: 12900.022 Da / Num. of mol.: 2 / Fragment: full length / Mutation: none Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PO4 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 39.5 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1.8 sodium-potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B1.2 / Detector: CCD / Date: Jul 3, 2001 |
Radiation | Monochromator: GRAPHITE / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 174182 / Num. obs: 15558 / % possible obs: 92.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 11.2 % / Biso Wilson estimate: 20 Å2 / Rmerge(I) obs: 0.036 / Rsym value: 0.036 / Net I/σ(I): 17 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 9.5 % / Rmerge(I) obs: 0.149 / Mean I/σ(I) obs: 3.1 / Num. unique all: 1353 / Rsym value: 0.149 / % possible all: 63 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: none Resolution: 1.9→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 32 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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