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- PDB-1jru: NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE) -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jru | ||||||
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Title | NMR STRUCTURE OF THE UBX DOMAIN FROM P47 (ENERGY MINIMISED AVERAGE) | ||||||
![]() | p47 protein | ||||||
![]() | UNKNOWN FUNCTION / UBIQUITIN SUPERFOLD / UBX / UNUSUAL N-TERMINAL FEATURE | ||||||
Function / homology | ![]() negative regulation of protein localization to centrosome / RHOH GTPase cycle / positive regulation of mitotic centrosome separation / nuclear membrane reassembly / Golgi stack / VCP-NSFL1C complex / spindle pole centrosome / Golgi organization / establishment of mitotic spindle orientation / autophagosome assembly ...negative regulation of protein localization to centrosome / RHOH GTPase cycle / positive regulation of mitotic centrosome separation / nuclear membrane reassembly / Golgi stack / VCP-NSFL1C complex / spindle pole centrosome / Golgi organization / establishment of mitotic spindle orientation / autophagosome assembly / ubiquitin binding / chromosome / ATPase binding / proteasome-mediated ubiquitin-dependent protein catabolic process / membrane fusion / lipid binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Model type details | minimized average | ||||||
![]() | Yuan, X.M. / Shaw, A. / Zhang, X.D. / Kondo, H. / Lally, J. / Freemont, P.S. / Matthews, S.J. | ||||||
![]() | ![]() Title: Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly. Authors: Yuan, X. / Shaw, A. / Zhang, X. / Kondo, H. / Lally, J. / Freemont, P.S. / Matthews, S. #1: ![]() Title: p47 is a cofactor for p97-mediated membrane fusion Authors: Kondo, H. / Rabouille, C. / Newman, R. / Levine, T.P. / Pappin, D. / Freemont, P.S. / Warren, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41.1 KB | Display | ![]() |
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PDB format | ![]() | 30.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 243 KB | Display | ![]() |
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Full document | ![]() | 242.8 KB | Display | |
Data in XML | ![]() | 4.5 KB | Display | |
Data in CIF | ![]() | 5.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 9901.343 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN (RESIDUES 282-370) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
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Sample preparation
Details | Contents: 1mM 13C, 15N-labelled p47 C-terminal domain sample in 20 mM NaAc, pH5.2 Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 5.2 / Pressure: 1 atm / Temperature: 302 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |