+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1jgn | ||||||
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タイトル | Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2 | ||||||
要素 |
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キーワード | RNA BINDING PROTEIN / all-helical domain / protein-peptide complex | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / mCRD-mediated mRNA stability complex / translation activator activity / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / Deadenylation of mRNA / positive regulation of cytoplasmic translation / poly(A) binding / M-decay: degradation of maternal mRNAs by maternally stored factors ...negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / mCRD-mediated mRNA stability complex / translation activator activity / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / Deadenylation of mRNA / positive regulation of cytoplasmic translation / poly(A) binding / M-decay: degradation of maternal mRNAs by maternally stored factors / regulatory ncRNA-mediated gene silencing / mRNA stabilization / poly(U) RNA binding / regulation of long-term synaptic potentiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Translation initiation complex formation / : / cell leading edge / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / positive regulation of viral genome replication / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / translation repressor activity / negative regulation of translational initiation / catalytic step 2 spliceosome / mRNA regulatory element binding translation repressor activity / AUF1 (hnRNP D0) binds and destabilizes mRNA / mRNA 3'-UTR binding / memory / Regulation of expression of SLITs and ROBOs / mRNA splicing, via spliceosome / cytoplasmic ribonucleoprotein granule / cytoplasmic stress granule / lamellipodium / spermatogenesis / negative regulation of translation / ribonucleoprotein complex / translation / focal adhesion / mRNA binding / RNA binding / extracellular exosome / membrane / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Kozlov, G. / Siddiqui, N. / Coillet-Matillon, S. / Ekiel, I. / Gehring, K. | ||||||
引用 | ジャーナル: EMBO J. / 年: 2004 タイトル: Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase 著者: Kozlov, G. / De Crescenzo, G. / Lim, N.S. / Siddiqui, N. / Fantus, D. / Kahvejian, A. / Trempe, J.F. / Elias, D. / Ekiel, I. / Sonenberg, N. / O'Connor-McCourt, M. / Gehring, K. #1: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 2001 タイトル: Structure and function of the C-terminal PABC domain of human poly(A)-binding protein 著者: Kozlov, G. / Trempe, J.F. / Khaleghpour, K. / Kahvejian, A. / Ekiel, I. / Gehring, K. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1jgn.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1jgn.ent.gz | 880.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1jgn.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1jgn_validation.pdf.gz | 356.3 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1jgn_full_validation.pdf.gz | 808.7 KB | 表示 | |
XML形式データ | 1jgn_validation.xml.gz | 88.6 KB | 表示 | |
CIF形式データ | 1jgn_validation.cif.gz | 113 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jg/1jgn ftp://data.pdbj.org/pub/pdb/validation_reports/jg/1jgn | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10347.936 Da / 分子数: 1 / 断片: C-terminal domain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PABPC1 / プラスミド: pGEX-6P-1 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21 GOLD / 参照: UniProt: P11940 |
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#2: タンパク質・ペプチド | 分子量: 2390.755 Da / 分子数: 1 / 断片: C-terminal 22 residues / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: pGEX-6P-1 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21 GOLD / 参照: UniProt: Q9BPZ3 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: The structure was determined using standard triple-resonance and homonuclear techniques |
-試料調製
詳細 |
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試料状態 | イオン強度: 0.1M NaCl / pH: 6.3 / 圧: ambient / 温度: 303 K | |||||||||||||||||||||
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: The structures are based on 2058 non-redundant NOE-derived distance constraints, 106 dihedral angle restraints, and 35 hydrogen bonds. | ||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 30 |