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- PDB-1jgn: Solution structure of the C-terminal PABC domain of human poly(A)... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jgn | ||||||
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Title | Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip2 | ||||||
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![]() | RNA BINDING PROTEIN / all-helical domain / protein-peptide complex | ||||||
Function / homology | ![]() gamma-glutamyltransferase / glutathione gamma-glutamate hydrolase / glutathione hydrolase activity / leukotriene C4 gamma-glutamyl transferase activity / glutathione catabolic process / glutathione biosynthetic process / regulation of long-term synaptic potentiation / translation repressor activity / negative regulation of translational initiation / mRNA regulatory element binding translation repressor activity ...gamma-glutamyltransferase / glutathione gamma-glutamate hydrolase / glutathione hydrolase activity / leukotriene C4 gamma-glutamyl transferase activity / glutathione catabolic process / glutathione biosynthetic process / regulation of long-term synaptic potentiation / translation repressor activity / negative regulation of translational initiation / mRNA regulatory element binding translation repressor activity / memory / spermatogenesis / negative regulation of translation / translation / mRNA binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Kozlov, G. / Siddiqui, N. / Coillet-Matillon, S. / Ekiel, I. / Gehring, K. | ||||||
![]() | ![]() Title: Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase Authors: Kozlov, G. / De Crescenzo, G. / Lim, N.S. / Siddiqui, N. / Fantus, D. / Kahvejian, A. / Trempe, J.F. / Elias, D. / Ekiel, I. / Sonenberg, N. / O'Connor-McCourt, M. / Gehring, K. #1: ![]() Title: Structure and function of the C-terminal PABC domain of human poly(A)-binding protein Authors: Kozlov, G. / Trempe, J.F. / Khaleghpour, K. / Kahvejian, A. / Ekiel, I. / Gehring, K. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1 MB | Display | ![]() |
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PDB format | ![]() | 880.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 356.3 KB | Display | ![]() |
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Full document | ![]() | 808.7 KB | Display | |
Data in XML | ![]() | 88.6 KB | Display | |
Data in CIF | ![]() | 113 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10347.936 Da / Num. of mol.: 1 / Fragment: C-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2390.755 Da / Num. of mol.: 1 / Fragment: C-terminal 22 residues Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using standard triple-resonance and homonuclear techniques |
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Sample preparation
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Sample conditions | Ionic strength: 0.1M NaCl / pH: 6.3 / Pressure: ambient / Temperature: 303 K | |||||||||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: The structures are based on 2058 non-redundant NOE-derived distance constraints, 106 dihedral angle restraints, and 35 hydrogen bonds. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 30 |