- PDB-1j53: Structure of the N-terminal Exonuclease Domain of the Epsilon Sub... -
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基本情報
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データベース: PDB / ID: 1j53
タイトル
Structure of the N-terminal Exonuclease Domain of the Epsilon Subunit of E.coli DNA Polymerase III at pH 8.5
要素
DNA polymerase III, epsilon chain
キーワード
TRANSFERASE / DNA polymerase proofreading domain
機能・相同性
機能・相同性情報
DNA polymerase III, core complex / DNA polymerase III complex / lagging strand elongation / replisome / exonuclease activity / DNA replication proofreading / leading strand elongation / 3'-5' exonuclease activity / DNA-templated DNA replication / DNA-directed DNA polymerase ...DNA polymerase III, core complex / DNA polymerase III complex / lagging strand elongation / replisome / exonuclease activity / DNA replication proofreading / leading strand elongation / 3'-5' exonuclease activity / DNA-templated DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA binding / metal ion binding / cytosol 類似検索 - 分子機能
DNA polymerase 3, epsilon subunit / DNA polymerase III epsilon subunit, exonuclease domain / Exonuclease / Exonuclease, RNase T/DNA polymerase III / EXOIII / Ribonuclease H-like superfamily/Ribonuclease H / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / 2-Layer Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
: / THYMIDINE-5'-PHOSPHATE / DNA polymerase III subunit epsilon 類似検索 - 構成要素
解像度: 1.8→1.86 Å / Rmerge(I) obs: 0.204 / Num. unique all: 1920 / % possible all: 98.4
反射
*PLUS
Rmerge(I) obs: 0.041
反射 シェル
*PLUS
Rmerge(I) obs: 0.204
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解析
ソフトウェア
名称
分類
DENZO
データ削減
SCALEPACK
データスケーリング
CCP4
モデル構築
CNS
精密化
CCP4
位相決定
精密化
解像度: 1.8→50 Å / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber 詳細: maximum likelihood target refinements (positional, individual B-factor, and simulated annealing using standard CNS scripts). The TMP 2100 molecule was fitted into the electron density but not ...詳細: maximum likelihood target refinements (positional, individual B-factor, and simulated annealing using standard CNS scripts). The TMP 2100 molecule was fitted into the electron density but not refined (as attempts to do so increased the Rfree value). The occupancies are zero.