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Open data
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Basic information
Entry | Database: PDB / ID: 1j36 | ||||||
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Title | Crystal Structure of Drosophila AnCE | ||||||
![]() | angiotensin converting enzyme | ||||||
![]() | HYDROLASE/HYDROLASE INHIBITOR / Angiotensin / HYDROLASE / HYDROLASE-HYDROLASE INHIBITOR complex | ||||||
Function / homology | ![]() Metabolism of Angiotensinogen to Angiotensins / metamorphosis / response to symbiotic bacterium / peptidyl-dipeptidase A / sexual reproduction / positive regulation of systemic arterial blood pressure / peptide hormone processing / regulation of systemic arterial blood pressure by renin-angiotensin / peptidyl-dipeptidase activity / carboxypeptidase activity ...Metabolism of Angiotensinogen to Angiotensins / metamorphosis / response to symbiotic bacterium / peptidyl-dipeptidase A / sexual reproduction / positive regulation of systemic arterial blood pressure / peptide hormone processing / regulation of systemic arterial blood pressure by renin-angiotensin / peptidyl-dipeptidase activity / carboxypeptidase activity / metallopeptidase activity / proteolysis / extracellular space / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, H.M. / Shin, D.R. / Yoo, O.J. / Lee, H. / Lee, J.-O. | ||||||
![]() | ![]() Title: Crystal structure of Drosophila angiotensin I-converting enzyme bound to captopril and lisinopril Authors: Kim, H.M. / Shin, D.R. / Yoo, O.J. / Lee, H. / Lee, J.-O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 262.1 KB | Display | ![]() |
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PDB format | ![]() | 208.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 520.1 KB | Display | ![]() |
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Full document | ![]() | 567 KB | Display | |
Data in XML | ![]() | 30.2 KB | Display | |
Data in CIF | ![]() | 46.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 70440.156 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.82 Å3/Da / Density % sol: 67.77 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Sodium Formate 7.2M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 22, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.928 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→20 Å / Num. all: 594157 / Num. obs: 547813 / % possible obs: 92.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.054 / Net I/σ(I): 22.1 |
Reflection shell | Resolution: 2.4→2.5 Å / Rmerge(I) obs: 0.233 / % possible all: 0.796 |
Reflection | *PLUS Num. obs: 82806 / Num. measured all: 547813 |
Reflection shell | *PLUS % possible obs: 79.6 % / Mean I/σ(I) obs: 4.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refinement | *PLUS Lowest resolution: 20 Å / Rfactor Rfree: 0.28 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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