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- PDB-1iz6: Crystal Structure of Translation Initiation Factor 5A from Pyroco... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1iz6 | ||||||
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Title | Crystal Structure of Translation Initiation Factor 5A from Pyrococcus Horikoshii | ||||||
![]() | Initiation Factor 5A | ||||||
![]() | BIOSYNTHETIC PROTEIN / SH3-like barrel / OB fold | ||||||
Function / homology | ![]() positive regulation of translational termination / positive regulation of translational elongation / translation elongation factor activity / translation initiation factor activity / ribosome binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yao, M. / Ohsawa, A. / Kikukawa, S. / Tanaka, I. / Kimura, M. | ||||||
![]() | ![]() Title: Crystal Structure of Hyperthermophilic Archaeal Initiation Factor 5A: A Homologue of Eukaryotic Initiation Factor 5A (eIF-5A) Authors: Yao, M. / Ohsawa, A. / Kikukawa, S. / Tanaka, I. / Kimura, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.3 KB | Display | ![]() |
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PDB format | ![]() | 72.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377.4 KB | Display | ![]() |
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Full document | ![]() | 387 KB | Display | |
Data in XML | ![]() | 10.2 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1bkbS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15305.637 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.1 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 4000, isopropanol, Hepers, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 20, 2001 |
Radiation | Monochromator: MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 25949 / % possible obs: 100 % / Observed criterion σ(I): 3 / Redundancy: 5.7 % / Biso Wilson estimate: 42.5 Å2 / Rmerge(I) obs: 0.081 / Rsym value: 0.074 / Net I/σ(I): 8.5 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 5.7 % / Rmerge(I) obs: 0.349 / Mean I/σ(I) obs: 2.4 / Num. unique all: 3842 / Rsym value: 0.317 / % possible all: 99.9 |
Reflection | *PLUS Lowest resolution: 100 Å / % possible obs: 100 % / Num. measured all: 148604 |
Reflection shell | *PLUS % possible obs: 99.9 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1BKB Resolution: 2→10 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: throughout / Bsol: 77.45 Å2 / ksol: 0.4885 e/Å3 | |||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 10 / % reflection obs: 100 %
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top | |||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 25746 / % reflection Rfree: 10 % | |||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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