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Open data
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Basic information
Entry | Database: PDB / ID: 1iul | ||||||
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Title | The structure of cell-free ID.343 from Thermus thermophilus | ||||||
![]() | hypothetical protein TT1466 | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | CoA binding domain / CoA binding domain / CoA-binding / NAD(P)-binding Rossmann-like Domain / NAD(P)-binding domain superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / CoA-binding domain-containing protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wada, T. / Shirouzu, M. / Park, S.-Y. / Tame, J.R. / Kuramitsu, S. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: Structure of a conserved CoA-binding protein synthesized by a cell-free system. Authors: Wada, T. / Shirouzu, M. / Terada, T. / Ishizuka, Y. / Matsuda, T. / Kigawa, T. / Kuramitsu, S. / Park, S.Y. / Tame, J.R. / Yokoyama, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 40.6 KB | Display | ![]() |
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PDB format | ![]() | 27.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.2 KB | Display | ![]() |
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Full document | ![]() | 420.3 KB | Display | |
Data in XML | ![]() | 8.6 KB | Display | |
Data in CIF | ![]() | 11.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15889.452 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 35.66 % | ||||||||||||||||||||||||||||||
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Crystal grow | Details: SODIUM ACETATE, AMMONIUM SULFATE, PEG MME 2000 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / pH: 4.9 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Sep 13, 2001 / Details: SI 111 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2→13 Å / Num. obs: 8037 / % possible obs: 98.9 % / Observed criterion σ(I): 0 / Redundancy: 3.9 % / Biso Wilson estimate: 16.7 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 21.8 | |||||||||||||||
Reflection shell | Resolution: 2→2.07 Å / Rmerge(I) obs: 0.097 / % possible all: 91.5 | |||||||||||||||
Reflection | *PLUS Lowest resolution: 13 Å / Num. measured all: 31394 | |||||||||||||||
Reflection shell | *PLUS % possible obs: 91.5 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 24.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→13 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.12 Å / Rfactor Rfree error: 0.04 / Total num. of bins used: 6
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Refinement | *PLUS Lowest resolution: 13 Å / % reflection Rfree: 5 % / Rfactor Rfree: 0.27 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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