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- PDB-1iqt: Solution structure of the C-terminal RNA-binding domain of hetero... -
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Basic information
Entry | Database: PDB / ID: 1iqt | |||||||||
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Title | Solution structure of the C-terminal RNA-binding domain of heterogeneous nuclear ribonucleoprotein D0 (AUF1) | |||||||||
![]() | heterogeneous nuclear ribonucleoprotein D0 | |||||||||
![]() | RNA BINDING PROTEIN / RNA-binding protein / hnRNP / AUF1 / telomere / DNA-binding protein | |||||||||
Function / homology | ![]() hepatocyte dedifferentiation / cellular response to putrescine / circadian regulation of translation / response to rapamycin / : / mCRD-mediated mRNA stability complex / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / response to sodium phosphate / 3'-UTR-mediated mRNA destabilization ...hepatocyte dedifferentiation / cellular response to putrescine / circadian regulation of translation / response to rapamycin / : / mCRD-mediated mRNA stability complex / negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / CRD-mediated mRNA stabilization / response to sodium phosphate / 3'-UTR-mediated mRNA destabilization / mRNA 3'-UTR AU-rich region binding / positive regulation of cytoplasmic translation / RNA catabolic process / regulation of telomere maintenance / telomeric DNA binding / minor groove of adenine-thymine-rich DNA binding / positive regulation of telomere capping / Processing of Capped Intron-Containing Pre-mRNA / cellular response to nitric oxide / RNA processing / response to electrical stimulus / mRNA Splicing - Major Pathway / cerebellum development / liver development / AUF1 (hnRNP D0) binds and destabilizes mRNA / cellular response to estradiol stimulus / positive regulation of translation / cellular response to amino acid stimulus / mRNA transcription by RNA polymerase II / regulation of circadian rhythm / histone deacetylase binding / response to calcium ion / regulation of gene expression / postsynaptic density / ribonucleoprotein complex / negative regulation of gene expression / glutamatergic synapse / chromatin binding / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / RNA binding / nucleoplasm / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
![]() | Katahira, M. / Miyanoiri, Y. / Enokizono, Y. / Matsuda, G. / Nagata, T. / Ishikawa, F. / Uesugi, S. | |||||||||
![]() | ![]() Title: Structure of the C-terminal RNA-binding domain of hnRNP D0 (AUF1), its interactions with RNA and DNA, and change in backbone dynamics upon complex formation with DNA. Authors: Katahira, M. / Miyanoiri, Y. / Enokizono, Y. / Matsuda, G. / Nagata, T. / Ishikawa, F. / Uesugi, S. #1: ![]() Title: Structure and Interactions with RNA of the N-Terminal UUAG-specific RNA-binding Domain of hnRNP D0 Authors: Nagata, T. / Kurihara, Y. / Matsuda, G. / Saeki, J. / Kohno, T. / Yanagida, Y. / Ishikawa, F. / Uesugi, S. / Katahira, M. #2: ![]() Title: Nuclear Proteins That Bind the Pre-mRNA 3' Splice Site Sequence r(UUAG/G) and the Human Telomeric DNA Sequence d(TTAGGG)n Authors: Ishikawa, F. / Matunis, M.J. / Dreyfuss, G. / Cech, T.R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 477.5 KB | Display | ![]() |
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PDB format | ![]() | 397.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 346.3 KB | Display | ![]() |
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Full document | ![]() | 529.7 KB | Display | |
Data in XML | ![]() | 61.2 KB | Display | |
Data in CIF | ![]() | 82.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 8657.043 Da / Num. of mol.: 1 / Fragment: C-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using 2D homonuclear and 3D 15N-edited heteronuclear techniques. |
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Sample preparation
Details |
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Sample conditions | Ionic strength: 0mM / pH: 6.0 / Pressure: 1 atm / Temperature: 298 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |