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- PDB-1iq5: Calmodulin/nematode CA2+/Calmodulin dependent kinase kinase fragment -
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Open data
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Basic information
Entry | Database: PDB / ID: 1iq5 | ||||||
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Title | Calmodulin/nematode CA2+/Calmodulin dependent kinase kinase fragment | ||||||
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![]() | METAL BINDING PROTEIN/PROTEIN BINDING / EF-hand / CALMODULIN / PROTEIN-PEPTIDE COMPLEX / METAL BINDING PROTEIN-PROTEIN BINDING COMPLEX | ||||||
Function / homology | ![]() CaMK IV-mediated phosphorylation of CREB / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / Activation of RAC1 downstream of NMDARs / positive regulation of CREB transcription factor activity / Ca2+/calmodulin-dependent protein kinase / calcium/calmodulin-dependent protein kinase activity / positive regulation of protein kinase activity / enzyme regulator activity / calcium-mediated signaling / calmodulin binding ...CaMK IV-mediated phosphorylation of CREB / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / Activation of RAC1 downstream of NMDARs / positive regulation of CREB transcription factor activity / Ca2+/calmodulin-dependent protein kinase / calcium/calmodulin-dependent protein kinase activity / positive regulation of protein kinase activity / enzyme regulator activity / calcium-mediated signaling / calmodulin binding / protein phosphorylation / signaling receptor binding / DNA-templated transcription / calcium ion binding / ATP binding / nucleus / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kurokawa, H. / Osawa, M. / Kurihara, H. / Katayama, N. / Tokumitsu, H. / Swindells, M.B. / Kainosho, M. / Ikura, M. | ||||||
![]() | ![]() Title: Target-induced conformational adaptation of calmodulin revealed by the crystal structure of a complex with nematode Ca(2+)/calmodulin-dependent kinase kinase peptide Authors: Kurokawa, H. / Osawa, M. / Kurihara, H. / Katayama, N. / Tokumitsu, H. / Swindells, M.B. / Kainosho, M. / Ikura, M. #1: ![]() Title: A novel target recognition revealed by calmodulin in complex with Ca2+-calmodulin-dependent kinase kinase Authors: Osawa, M. / Tokumitsu, H. / Swindells, M.B. / Kurihara, H. / Orita, M. / Shibanuma, T. / Furuya, T. / Ikura, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.9 KB | Display | ![]() |
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PDB format | ![]() | 33.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16852.545 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Protein/peptide | Mass: 3198.920 Da / Num. of mol.: 1 / Fragment: CALMODULIN BINDING DOMAIN (RESIDUES 331-357) / Source method: obtained synthetically Details: This sequence occurs naturally in Caenorhabditis elegans References: UniProt: Q8T8D4, UniProt: Q3Y416*PLUS, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor | ||
#3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.81 % | ||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: ammonium sulfate, citratet, sodium tartrae, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: CUSTOM-MADE / Detector: IMAGE PLATE / Date: Feb 10, 1999 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→100 Å / Num. all: 57477 / Num. obs: 17155 / % possible obs: 94.6 % / Observed criterion σ(I): 2 / Redundancy: 3 % / Biso Wilson estimate: 25.798 Å2 / Rmerge(I) obs: 0.024 / Net I/σ(I): 21.9 |
Reflection shell | Resolution: 1.8→1.91 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.212 / % possible all: 89.1 |
Reflection | *PLUS Lowest resolution: 100 Å / Num. measured all: 57477 |
Reflection shell | *PLUS % possible obs: 89.1 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.8→100 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 100 Å / Rfactor obs: 0.217 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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