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Yorodumi- PDB-1iid: Crystal Structure of Saccharomyces cerevisiae N-myristoyltransfer... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1iid | ||||||
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Title | Crystal Structure of Saccharomyces cerevisiae N-myristoyltransferase with Bound S-(2-oxo)pentadecylCoA and the Octapeptide GLYASKLA | ||||||
Components |
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Keywords | TRANSFERASE | ||||||
Function / homology | Function and homology information Inactivation, recovery and regulation of the phototransduction cascade / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Farazi, T.A. / Gordon, J.I. / Waksman, G. | ||||||
Citation | Journal: Biochemistry / Year: 2001 Title: Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis. Authors: Farazi, T.A. / Waksman, G. / Gordon, J.I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1iid.cif.gz | 106.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1iid.ent.gz | 79.2 KB | Display | PDB format |
PDBx/mmJSON format | 1iid.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ii/1iid ftp://data.pdbj.org/pub/pdb/validation_reports/ii/1iid | HTTPS FTP |
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-Related structure data
Related structure data | 1iicC 2nmtS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 49013.973 Da / Num. of mol.: 1 Fragment: N-myristoyltransferase (N-terminal 33 residues deleted) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: NMT / Plasmid: pBB502 / Production host: Escherichia coli (E. coli) / Strain (production host): JM101 References: UniProt: P14743, glycylpeptide N-tetradecanoyltransferase | ||||
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#2: Protein/peptide | Mass: 822.970 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: This peptide was chemically synthesized. | ||||
#3: Chemical | #4: Chemical | ChemComp-NHM / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 54 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: PEG 2000 MME, nickel chloride, sodium cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Oct 11, 2000 / Details: Yale Mirrors |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→30 Å / Num. all: 18292 / Num. obs: 17552 / % possible obs: 96 % / Observed criterion σ(F): 2 / Biso Wilson estimate: 43.6 Å2 / Rsym value: 5.4 |
Reflection shell | Resolution: 2.5→2.59 Å / % possible all: 87.21 |
Reflection | *PLUS Num. measured all: 225123 / Rmerge(I) obs: 0.054 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2NMT Resolution: 2.5→29.72 Å / Rfactor Rfree error: 0.01 / Data cutoff high absF: 454763.73 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.04 Å2 / ksol: 0.352 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→29.72 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.036 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 2 / % reflection Rfree: 4.8 % | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 55.2 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.396 / % reflection Rfree: 4.5 % / Rfactor Rwork: 0.353 |