[English] 日本語
Yorodumi- PDB-1igi: 26-10 FAB:DIGOXIN COMPLEX-AFFINITY AND SPECIFICITY DUE TO SURFACE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1igi | ||||||
---|---|---|---|---|---|---|---|
Title | 26-10 FAB:DIGOXIN COMPLEX-AFFINITY AND SPECIFICITY DUE TO SURFACE COMPLEMENTARITY | ||||||
Components |
| ||||||
Keywords | IMMUNOGLOBULIN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : / : Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.7 Å | ||||||
Authors | Jeffrey, P.D. / Sheriff, S. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: 26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity. Authors: Jeffrey, P.D. / Strong, R.K. / Sieker, L.C. / Chang, C.Y. / Campbell, R.L. / Petsko, G.A. / Haber, E. / Margolies, M.N. / Sheriff, S. #1: Journal: J.Mol.Biol. / Year: 1983 Title: Crystallization of the Fab Fragment of a Monoclonal Anti-Digoxin Antibody and its Complex with Digoxin Authors: Rose, D.R. / Seaton, B.A. / Petsko, G.A. / Novotny, J. / Margolies, M.N. / Locke, E. / Haber, E. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1igi.cif.gz | 93 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1igi.ent.gz | 71.3 KB | Display | PDB format |
PDBx/mmJSON format | 1igi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1igi_validation.pdf.gz | 424.6 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1igi_full_validation.pdf.gz | 436.8 KB | Display | |
Data in XML | 1igi_validation.xml.gz | 17.6 KB | Display | |
Data in CIF | 1igi_validation.cif.gz | 23.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/1igi ftp://data.pdbj.org/pub/pdb/validation_reports/ig/1igi | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: RESIDUES 8, 95, 141 OF THE L CHAIN AND 149, 151, 200 OF THE H CHAIN ARE CIS PROLINES. |
-Components
#1: Antibody | Mass: 24057.676 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Strain: A/J / References: PIR: S52028 |
---|---|
#2: Antibody | Mass: 23307.027 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Strain: A/J / References: PIR: S38950 |
Sequence details | THE FAB FRAGMENT IS NUMBERED BY THE CONVENTION OF E.KABAT (E.A.KABAT,T.T.WU,H.M.PERRY,K.S. ...THE FAB FRAGMENT IS NUMBERED BY THE CONVENTION |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.37 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 7.5 / Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.7 Å / Num. obs: 7998 / % possible obs: 61 % / Num. measured all: 16046 / Rmerge(I) obs: 0.056 |
-Processing
Software | Name: X-PLOR / Classification: refinement | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.7→8 Å / Rfactor Rwork: 0.177 / σ(F): 1 Details: THE FOLLOWING REGIONS OF THE HEAVY CHAIN (H) WERE INCLUDED IN THE MODEL BUT HAVE POORLY DEFINED ELECTRON DENSITY: 25 - 30, 53 - 56, AND 128 - 137. | |||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→8 Å
| |||||||||||||||
Refine LS restraints |
| |||||||||||||||
Software | *PLUS Name: X-PLOR / Classification: refinement | |||||||||||||||
Refinement | *PLUS Num. reflection obs: 7326 / Rfactor Rwork: 0.177 | |||||||||||||||
Solvent computation | *PLUS | |||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||
Refine LS restraints | *PLUS
|