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Yorodumi- PDB-1igc: IGG1 FAB FRAGMENT (MOPC21) COMPLEX WITH DOMAIN III OF PROTEIN G F... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1igc | ||||||
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Title | IGG1 FAB FRAGMENT (MOPC21) COMPLEX WITH DOMAIN III OF PROTEIN G FROM STREPTOCOCCUS | ||||||
Components |
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Keywords | COMPLEX (ANTIBODY/BINDING PROTEIN) / PROTEIN G / STREPTOCOCCUS / COMPLEX (ANTIBODY-BINDING PROTEIN) COMPLEX | ||||||
Function / homology | Function and homology information immunoglobulin complex / IgG binding / adaptive immune response / immune response / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Streptococcus sp. G148 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.6 Å | ||||||
Authors | Derrick, J.P. / Wigley, D.B. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1994 Title: The third IgG-binding domain from streptococcal protein G. An analysis by X-ray crystallography of the structure alone and in a complex with Fab. Authors: Derrick, J.P. / Wigley, D.B. #1: Journal: Biochemistry / Year: 1994 Title: Two Crystal Structures of the B1 Immunoglobulin-Binding Domain of Streptococcal Protein G and Comparison with NMR Authors: Gallagher, T. / Alexander, P. / Bryan, P. / Gilliland, G.L. #2: Journal: Nature / Year: 1992 Title: Crystal Structure of a Streptococcal Protein G Domain Bound to an Fab Fragment Authors: Derrick, J.P. / Wigley, D.B. #3: Journal: J.Mol.Biol. / Year: 1992 Title: Crystallization and Preliminary Analysis of the Complex between a Mouse Fab Fragment and a Single Igg-Binding Domain from Streptococcal Protein G Authors: Derrick, J.P. / Davies, G.J. / Dauter, Z. / Wilson, K.S. / Wigley, D.B. #4: Journal: J.Mol.Biol. / Year: 1992 Title: Determination of the Solution Structures of Domains II and III of Protein G from Streptococcus by 1H Nuclear Magnetic Resonance Authors: Lian, L.-Y. / Derrick, J.P. / Sutcliff, M.J. / Yang, J.C. / Roberts, G.C.K. #5: Journal: Biochemistry / Year: 1992 Title: 1.67 Angstroms X-Ray Structure of the B2 Immunoglobulin-Binding Domain of Streptococcal Protein G and Comparison to the NMR Structure of the B1 Domain Authors: Achari, A. / Hale, S.P. / Howard, A.J. / Clore, G.M. / Gronenborn, A.M. / Hardman, K.D. / Whitlow, M. #6: Journal: Science / Year: 1991 Title: A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein G Authors: Gronenborn, A.M. / Filpula, D.R. / Essig, N.Z. / Achari, A. / Whitlow, M. / Wingfield, P.T. / Clore, G.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1igc.cif.gz | 119.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1igc.ent.gz | 90.6 KB | Display | PDB format |
PDBx/mmJSON format | 1igc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1igc_validation.pdf.gz | 440 KB | Display | wwPDB validaton report |
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Full document | 1igc_full_validation.pdf.gz | 506 KB | Display | |
Data in XML | 1igc_validation.xml.gz | 32.5 KB | Display | |
Data in CIF | 1igc_validation.cif.gz | 44.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/1igc ftp://data.pdbj.org/pub/pdb/validation_reports/ig/1igc | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO L 8 / 2: CIS PROLINE - PRO L 95 / 3: CIS PROLINE - PRO H 103 / 4: CIS PROLINE - PRO H 154 / 5: CIS PROLINE - PRO H 156 |
-Components
#1: Antibody | Mass: 23556.004 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): K12 JM101 / References: UniProt: P01634 |
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#2: Antibody | Mass: 23856.750 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: PUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): K12 JM101 / References: UniProt: P01783 |
#3: Protein | Mass: 6657.354 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus sp. G148 (bacteria) / Plasmid: PUC18 / Production host: Escherichia coli (E. coli) / Strain (production host): K12 JM101 / References: UniProt: P06654 |
#4: Water | ChemComp-HOH / |
Sequence details | THERE ARE NO RESIDUES MISSING FROM THE LIGHT CHAIN. THERE ARE NO RESIDUES MISSING FROM THE N ...THERE ARE NO RESIDUES MISSING FROM THE LIGHT CHAIN. THERE ARE NO RESIDUES MISSING FROM THE N TERMINUS OF THE HEAVY CHAIN. THE SITE OF PROTEOLYSI |
Source details | MOPC21 (IGG1 KAPPA) WAS ISOLATED FROM ASCITES FLUID, GENERATED BY A MINERAL OIL-INDUCED ...MOPC21 (IGG1 KAPPA) WAS ISOLATED FROM ASCITES FLUID, GENERATED BY A MINERAL OIL-INDUCED PLASMACYTO |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.26 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 4.8 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Wavelength: 1 Å |
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Detector | Date: Apr 1, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Num. obs: 17204 / % possible obs: 98.7 % / Rmerge(I) obs: 0.079 |
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 20 Å / Rmerge(I) obs: 0.079 |
-Processing
Software |
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Refinement | Resolution: 2.6→20 Å / Rfactor obs: 0.168 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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