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- PDB-1iba: GLUCOSE PERMEASE (DOMAIN IIB), NMR, 11 STRUCTURES -

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Basic information

Entry
Database: PDB / ID: 1iba
TitleGLUCOSE PERMEASE (DOMAIN IIB), NMR, 11 STRUCTURES
ComponentsGLUCOSE PERMEASE
KeywordsPHOSPHOTRANSFERASE / PHOSPHOTRANSFERASE SYSTEM / SUGAR TRANSPORT / TRANSFERASE / PHOSPHORYLATION / TRANSMEMBRANE / INNER MEMBRANE
Function / homology
Function and homology information


protein-phosphocysteine-glucose phosphotransferase system transporter activity / protein-Npi-phosphohistidine-D-glucose phosphotransferase / protein-N(PI)-phosphohistidine-sugar phosphotransferase activity / D-glucose import across plasma membrane / D-glucose transmembrane transporter activity / D-glucose transmembrane transport / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / kinase activity / regulation of DNA-templated transcription ...protein-phosphocysteine-glucose phosphotransferase system transporter activity / protein-Npi-phosphohistidine-D-glucose phosphotransferase / protein-N(PI)-phosphohistidine-sugar phosphotransferase activity / D-glucose import across plasma membrane / D-glucose transmembrane transporter activity / D-glucose transmembrane transport / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / kinase activity / regulation of DNA-templated transcription / membrane / plasma membrane
Similarity search - Function
Glucose permease domain IIB / Phosphotransferase system, maltose/glucose-specific subfamily IIC component / PTS system glucose-specific IIBC component / : / Phosphotransferase system, IIB component, type 1 / Phosphotransferase system, EIIC component, type 1 / Phosphotransferase system EIIB, cysteine phosphorylation site / Glucose permease domain IIB / phosphotransferase system, EIIB / PTS EIIB domains cysteine phosphorylation site signature. ...Glucose permease domain IIB / Phosphotransferase system, maltose/glucose-specific subfamily IIC component / PTS system glucose-specific IIBC component / : / Phosphotransferase system, IIB component, type 1 / Phosphotransferase system, EIIC component, type 1 / Phosphotransferase system EIIB, cysteine phosphorylation site / Glucose permease domain IIB / phosphotransferase system, EIIB / PTS EIIB domains cysteine phosphorylation site signature. / PTS_EIIB type-1 domain profile. / PTS_EIIC type-1 domain profile. / Phosphotransferase system, EIIC / Phosphotransferase system, EIIC / Gyrase A; domain 2 / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
PTS system glucose-specific EIICB component
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodSOLUTION NMR
AuthorsEberstadt, M. / Grdadolnik, S.G. / Gemmecker, G. / Kessler, H. / Buhr, A. / Erni, B.
Citation
Journal: Biochemistry / Year: 1996
Title: Solution structure of the IIB domain of the glucose transporter of Escherichia coli.
Authors: Eberstadt, M. / Grdadolnik, S.G. / Gemmecker, G. / Kessler, H. / Buhr, A. / Erni, B.
#1: Journal: Eur.J.Biochem. / Year: 1994
Title: The Glucose Transporter of Escherichia Coli. Assignment of the 1H, 13C and 15N Resonances and Identification of the Secondary Structure of the Soluble Iib Domain
Authors: Golic Grdadolnik, S. / Eberstadt, M. / Gemmecker, G. / Kessler, H. / Buhr, A. / Erni, B.
#2: Journal: J.Biol.Chem. / Year: 1994
Title: The Glucose Transporter of Escherichia Coli
Authors: Buhr, A. / Flukiger, K. / Erni, B.
#3: Journal: J.Biol.Chem. / Year: 1993
Title: Cysteine Phosphorylation of the Glucose Transporter of Escherichia Coli
Authors: Meins, M. / Jeno, P. / Muller, D. / Richter, W.J. / Rosenbusch, J.P. / Erni, B.
#4: Journal: J.Biol.Chem. / Year: 1986
Title: Glucose-Permease of the Bacterial Phosphotransferase System. Gene Cloning, Overproduction, and Amino Acid Sequence of Enzyme Iiglc
Authors: Erni, B. / Zanolari, B.
History
DepositionMar 23, 1996Processing site: BNL
Revision 1.0Oct 14, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4May 1, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: GLUCOSE PERMEASE


Theoretical massNumber of molelcules
Total (without water)10,7521
Polymers10,7521
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)11 / -
Representative

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Components

#1: Protein GLUCOSE PERMEASE


Mass: 10752.142 Da / Num. of mol.: 1 / Fragment: DOMAIN IIB
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K-12, W3110 / Plasmid: PQBH / Gene (production host): PTSG / Production host: Escherichia coli (E. coli)
References: UniProt: P69786, protein-Npi-phosphohistidine-sugar phosphotransferase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

NMR softwareName: DIANA / Version: 2.8 / Developer: GUNTERT, BRAUN, WUTHRICH / Classification: refinement
NMR ensembleConformers submitted total number: 11

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