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- PDB-1ias: CYTOPLASMIC DOMAIN OF UNPHOSPHORYLATED TYPE I TGF-BETA RECEPTOR C... -

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Basic information

Entry
Database: PDB / ID: 1ias
TitleCYTOPLASMIC DOMAIN OF UNPHOSPHORYLATED TYPE I TGF-BETA RECEPTOR CRYSTALLIZED WITHOUT FKBP12
ComponentsTGF-BETA RECEPTOR TYPE I
KeywordsTRANSFERASE / kinase / TGF-beta receptor / GS region
Function / homology
Function and homology information


extracellular structure organization / epicardium morphogenesis / vascular endothelial cell proliferation / parathyroid gland development / regulation of cardiac muscle cell proliferation / transforming growth factor beta ligand-receptor complex / positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation / myofibroblast differentiation / transforming growth factor beta receptor activity / TGFBR2 Kinase Domain Mutants in Cancer ...extracellular structure organization / epicardium morphogenesis / vascular endothelial cell proliferation / parathyroid gland development / regulation of cardiac muscle cell proliferation / transforming growth factor beta ligand-receptor complex / positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation / myofibroblast differentiation / transforming growth factor beta receptor activity / TGFBR2 Kinase Domain Mutants in Cancer / trophoblast cell migration / pharyngeal system development / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / angiogenesis involved in coronary vascular morphogenesis / positive regulation of mesenchymal stem cell proliferation / ventricular compact myocardium morphogenesis / positive regulation of tight junction disassembly / positive regulation of extracellular matrix assembly / cardiac epithelial to mesenchymal transition / neuron fate commitment / mesenchymal cell differentiation / transforming growth factor beta receptor activity, type I / TGFBR3 regulates TGF-beta signaling / positive regulation of vasculature development / activin receptor activity, type I / activin receptor complex / primordial germ cell migration / regulation of epithelial to mesenchymal transition / anterior/posterior pattern specification / receptor protein serine/threonine kinase / type II transforming growth factor beta receptor binding / activin binding / embryonic cranial skeleton morphogenesis / transmembrane receptor protein serine/threonine kinase activity / TGFBR1 LBD Mutants in Cancer / ventricular trabecula myocardium morphogenesis / coronary artery morphogenesis / activin receptor signaling pathway / artery morphogenesis / collagen fibril organization / negative regulation of chondrocyte differentiation / skeletal system morphogenesis / response to cholesterol / transforming growth factor beta binding / I-SMAD binding / endothelial cell activation / roof of mouth development / ventricular septum morphogenesis / thymus development / SMAD binding / TGF-beta receptor signaling activates SMADs / positive regulation of SMAD protein signal transduction / regulation of protein ubiquitination / epithelial to mesenchymal transition / skeletal system development / bicellular tight junction / cellular response to transforming growth factor beta stimulus / transforming growth factor beta receptor signaling pathway / positive regulation of stress fiber assembly / positive regulation of epithelial to mesenchymal transition / kidney development / positive regulation of endothelial cell proliferation / in utero embryonic development / positive regulation of apoptotic signaling pathway / negative regulation of cell migration / cell motility / negative regulation of extrinsic apoptotic signaling pathway / wound healing / Downregulation of TGF-beta receptor signaling / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / peptidyl-serine phosphorylation / cellular response to growth factor stimulus / heart development / regulation of gene expression / nervous system development / UCH proteinases / positive regulation of cell growth / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / regulation of cell cycle / protein kinase activity / signaling receptor complex / endosome / intracellular signal transduction / Ub-specific processing proteases / positive regulation of cell migration / membrane raft / ubiquitin protein ligase binding / protein serine/threonine kinase activity / positive regulation of gene expression / positive regulation of cell population proliferation / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / signal transduction / cell surface / ATP binding / membrane / nucleus / plasma membrane
Similarity search - Function
GS domain / Transforming growth factor beta type I GS-motif / GS domain profile. / GS motif / Activin types I and II receptor domain / Activin types I and II receptor domain / Ser/Thr protein kinase, TGFB receptor / Snake toxin-like superfamily / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 ...GS domain / Transforming growth factor beta type I GS-motif / GS domain profile. / GS motif / Activin types I and II receptor domain / Activin types I and II receptor domain / Ser/Thr protein kinase, TGFB receptor / Snake toxin-like superfamily / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
TGF-beta receptor type-1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsHuse, M. / Muir, T.W. / Chen, Y.-G. / Kuriyan, J. / Massague, J.
CitationJournal: Mol.Cell / Year: 2001
Title: The TGF beta receptor activation process: an inhibitor- to substrate-binding switch.
Authors: Huse, M. / Muir, T.W. / Xu, L. / Chen, Y.G. / Kuriyan, J. / Massague, J.
History
DepositionMar 23, 2001Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 3, 2001Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 7, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.4Apr 3, 2024Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: TGF-BETA RECEPTOR TYPE I
B: TGF-BETA RECEPTOR TYPE I
C: TGF-BETA RECEPTOR TYPE I
D: TGF-BETA RECEPTOR TYPE I
E: TGF-BETA RECEPTOR TYPE I
hetero molecules


Theoretical massNumber of molelcules
Total (without water)196,04420
Polymers194,6035
Non-polymers1,44115
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)172.560, 251.617, 140.228
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number20
Space group name H-MC2221

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Components

#1: Protein
TGF-BETA RECEPTOR TYPE I


Mass: 38920.641 Da / Num. of mol.: 5 / Fragment: CYTOPLASMIC DOMAIN (RESIDUES 162-503)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P36897, EC: 2.7.1.37
#2: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: SO4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.91 Å3/Da / Density % sol: 68.54 %
Description: While TbR-I does crystallize in the absence of FKBP12, the crystals diffract poorly and are difficult to handle. To circumvent this problem, we sought high affinity, small molecule ...Description: While TbR-I does crystallize in the absence of FKBP12, the crystals diffract poorly and are difficult to handle. To circumvent this problem, we sought high affinity, small molecule inhibitors that might stabilize TbR-I and thereby allow growth of better crystals. High affinity inhibitors of this class of kinases are not widely available in the public domain. We were provided with a quinazoline derivative that specifically inhibits TbR-I with an IC50 significantly less than 10mM (NPC-30345, proprietary to Scios, Inc., a company not affiliated with any of the authors). Addition of NPC-30345 allows the TbR-I crystals to grow larger and to diffract X-rays to moderate resolution using synchrotron sources. It is not possible to build a detailed molecular model for the inhibitor at the resolution of the analysis without knowledge of its precise chemical structure, which is proprietary to Scios, Inc. As such, we have not included a model for the inhibitor in the refinement. Electron density maps calculated using structure factors (deposited) and the model allow visualization of density for the inhibitor.
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.8
Details: 100 mM MES pH 5.8, 2.4 M ammonium sulfate, 10 % glycerol v/v, 150 mM NaCl, 1 mM NPC-30345, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystal
*PLUS
Density % sol: 70 %
Crystal grow
*PLUS
Temperature: 4 ℃ / Method: vapor diffusion / PH range low: 5.8 / PH range high: 5.7
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-ID
1100 mMMES1reservoir
21.95-2.05 Mammonium sulfate1reservoir

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.948 Å
DetectorType: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 27, 2001
RadiationMonochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.948 Å / Relative weight: 1
ReflectionResolution: 2.9→50 Å / Num. all: 66462 / Num. obs: 59616 / % possible obs: 89.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 57.4 Å2 / Rmerge(I) obs: 0.08 / Rsym value: 0.08 / Net I/σ(I): 22
Reflection shellResolution: 2.9→30 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.312 / Mean I/σ(I) obs: 3.5 / Num. unique all: 2677 / Rsym value: 0.312 / % possible all: 61
Reflection
*PLUS
Lowest resolution: 50 Å / Rmerge(I) obs: 0.08
Reflection shell
*PLUS
% possible obs: 61.1 %

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Processing

Software
NameClassification
AMoREphasing
CNSrefinement
DENZOdata reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: TGF-beta receptor from TGF-beta receptor/FKBP12 structure

Resolution: 2.9→30 Å / Cross valid method: FREE R-VALUE / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflection% reflectionSelection details
Rfree0.284 5833 -10 % randomly selected
Rwork0.255 ---
all0.255 57436 --
obs0.255 51603 85.9 %-
Displacement parametersBiso mean: 68.8 Å2
Baniso -1Baniso -2Baniso -3
1--23.53 Å20 Å20 Å2
2--32.62 Å20 Å2
3----9.09 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.52 Å0.45 Å
Luzzati d res low-5 Å
Luzzati sigma a0.78 Å0.65 Å
Refinement stepCycle: LAST / Resolution: 2.9→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13106 0 75 0 13181
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_angle_deg1.3
X-RAY DIFFRACTIONc_dihedral_angle_d21.2
X-RAY DIFFRACTIONc_improper_angle_d0.76
X-RAY DIFFRACTIONc_bond_d0.009
Software
*PLUS
Name: CNS / Classification: refinement
Refinement
*PLUS
Highest resolution: 2.9 Å / Lowest resolution: 30 Å / σ(F): 0 / % reflection Rfree: 10 %
Solvent computation
*PLUS
Displacement parameters
*PLUS
Biso mean: 68.8 Å2
Refine LS restraints
*PLUS
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_angle_deg1.3
X-RAY DIFFRACTIONc_dihedral_angle_d
X-RAY DIFFRACTIONc_dihedral_angle_deg21.2
X-RAY DIFFRACTIONc_improper_angle_d
X-RAY DIFFRACTIONc_improper_angle_deg0.76

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