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Open data
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Basic information
Entry | Database: PDB / ID: 1i8t | ||||||
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Title | STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI | ||||||
![]() | UDP-GALACTOPYRANOSE MUTASE | ||||||
![]() | ISOMERASE / Rossmann Fold / FAD / UDP-galactopyranose / mutase / contractase | ||||||
Function / homology | ![]() UDP-galactopyranose mutase / UDP-galactopyranose mutase activity / lipopolysaccharide biosynthetic process / flavin adenine dinucleotide binding / protein homodimerization activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sanders, D.A.R. / Staines, A.G. / McMahon, S.A. / McNeil, M.R. / Whitfield, C. / Naismith, J.H. | ||||||
![]() | ![]() Title: UDP-galactopyranose mutase has a novel structure and mechanism. Authors: Sanders, D.A. / Staines, A.G. / McMahon, S.A. / McNeil, M.R. / Whitfield, C. / Naismith, J.H. #1: ![]() Title: Molecular Placement of Experimental Electron Density: A Case Study on UDP-Galactopyranose Mutase Authors: Sanders, D.A.R. / McMahon, S.A. / Leonard, G.L. / Naimsith, J.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.4 KB | Display | ![]() |
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PDB format | ![]() | 133.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43025.789 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.48 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: PEG 4K, 0.01 mM l-cystein, Hepe, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: vapor diffusionDetails: used macroseeding, Sanders, D.A.R., (2001) Acta Crystallogr., Sect.D, 57, 1415. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 28, 2000 | |||||||||||||||
Radiation |
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Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 2.4→40 Å / Num. all: 28865 / Num. obs: 28288 / % possible obs: 98 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.9 % / Biso Wilson estimate: 37 Å2 / Rmerge(I) obs: 0.059 / Net I/σ(I): 10.9 | |||||||||||||||
Reflection shell | Resolution: 2.4→2.52 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.224 / Mean I/σ(I) obs: 3.3 / % possible all: 93 | |||||||||||||||
Reflection | *PLUS Lowest resolution: 40 Å / % possible obs: 98 % | |||||||||||||||
Reflection shell | *PLUS % possible obs: 93 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.4→40 Å
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LS refinement shell | Resolution: 2.4→2.52 Å | ||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 40 Å / σ(F): 2 / % reflection Rfree: 5 % / Rfactor obs: 0.185 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.4 Å |