+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1i8h | ||||||
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タイトル | SOLUTION STRUCTURE OF PIN1 WW DOMAIN COMPLEXED WITH HUMAN TAU PHOSPHOTHREONINE PEPTIDE | ||||||
要素 |
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キーワード | MEMBRANE PROTEIN/ISOMERASE (生体膜) / CYTOSKELETON (細胞骨格) / NUCLEAR PROTEIN / MEMBRANE PROTEIN-ISOMERASE COMPLEX (生体膜) | ||||||
機能・相同性 | 機能・相同性情報 cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / plus-end-directed organelle transport along microtubule / 軸索輸送 / histone-dependent DNA binding / neurofibrillary tangle assembly ...cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / plus-end-directed organelle transport along microtubule / 軸索輸送 / histone-dependent DNA binding / neurofibrillary tangle assembly / positive regulation of diacylglycerol kinase activity / postsynaptic cytosol / negative regulation of establishment of protein localization to mitochondrion / neurofibrillary tangle / positive regulation of protein localization to synapse / mitogen-activated protein kinase kinase binding / microtubule lateral binding / tubulin complex / phosphatidylinositol bisphosphate binding / main axon / regulation of mitotic nuclear division / regulation of long-term synaptic depression / negative regulation of kinase activity / negative regulation of tubulin deacetylation / generation of neurons / regulation of chromosome organization / positive regulation of protein localization / rRNA metabolic process / internal protein amino acid acetylation / regulation of mitochondrial fission / intracellular distribution of mitochondria / negative regulation of SMAD protein signal transduction / axonal transport of mitochondrion / axon development / central nervous system neuron development / regulation of microtubule polymerization / PI5P Regulates TP53 Acetylation / 微小管 / minor groove of adenine-thymine-rich DNA binding / negative regulation of amyloid-beta formation / lipoprotein particle binding / cytoskeletal motor activity / dynactin binding / glial cell projection / negative regulation of mitochondrial membrane potential / apolipoprotein binding / protein polymerization / negative regulation of mitochondrial fission / RHO GTPases Activate NADPH Oxidases / axolemma / phosphoserine residue binding / Caspase-mediated cleavage of cytoskeletal proteins / regulation of microtubule polymerization or depolymerization / positive regulation of axon extension / protein peptidyl-prolyl isomerization / regulation of microtubule cytoskeleton organization / supramolecular fiber organization / Activation of AMPK downstream of NMDARs / stress granule assembly / cytoplasmic microtubule organization / regulation of cellular response to heat / axon cytoplasm / positive regulation of protein dephosphorylation / regulation of calcium-mediated signaling / positive regulation of microtubule polymerization / cellular response to brain-derived neurotrophic factor stimulus / somatodendritic compartment / synapse assembly / phosphatidylinositol binding / nuclear periphery / cellular response to nerve growth factor stimulus / positive regulation of superoxide anion generation / ciliary basal body / protein phosphatase 2A binding / regulation of cytokinesis / negative regulation of protein binding / プロリルイソメラーゼ / regulation of autophagy / peptidyl-prolyl cis-trans isomerase activity / Negative regulators of DDX58/IFIH1 signaling / astrocyte activation / phosphoprotein binding / response to lead ion / synapse organization / regulation of protein phosphorylation / negative regulation of transforming growth factor beta receptor signaling pathway / microglial cell activation / Hsp90 protein binding / regulation of synaptic plasticity / regulation of protein stability / PKR-mediated signaling / tau protein binding / protein homooligomerization / negative regulation of ERK1 and ERK2 cascade / negative regulation of protein catabolic process / neuron differentiation / cytoplasmic ribonucleoprotein granule / 記憶 / ISG15 antiviral mechanism / microtubule cytoskeleton organization 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / distance geometry simulated annealing | ||||||
データ登録者 | Wintjens, R. / Wieruszeski, J.-M. / Drobecq, H. / Lippens, G. / Landrieu, I. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2001 タイトル: 1H NMR study on the binding of Pin1 Trp-Trp domain with phosphothreonine peptides. 著者: Wintjens, R. / Wieruszeski, J.M. / Drobecq, H. / Rousselot-Pailley, P. / Buee, L. / Lippens, G. / Landrieu, I. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1i8h.cif.gz | 168.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1i8h.ent.gz | 145.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1i8h.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/i8/1i8h ftp://data.pdbj.org/pub/pdb/validation_reports/i8/1i8h | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 1464.623 Da / 分子数: 1 / 断片: (RESIDUES 541-553) / 由来タイプ: 合成 詳細: The ligand phosphopeptide was synthesized from Rink amide resin using Fmoc strategy and activation by HBTU and HOBT in a 431A peptide synthesizer. The sequence of the peptide is naturally ...詳細: The ligand phosphopeptide was synthesized from Rink amide resin using Fmoc strategy and activation by HBTU and HOBT in a 431A peptide synthesizer. The sequence of the peptide is naturally found in Homo sapiens (Human). 参照: UniProt: P10636 |
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#2: タンパク質・ペプチド | 分子量: 4462.899 Da / 分子数: 1 / 断片: WW DOMAIN (RESIDUES 6-44) / 由来タイプ: 合成 詳細: The Pin1 WW domain was obtained by peptide synthesis using the BOC-benzyl strategy and the HBTU in situ activation protocol on an Applied 430A peptide synthesizer. The sequence of the peptide ...詳細: The Pin1 WW domain was obtained by peptide synthesis using the BOC-benzyl strategy and the HBTU in situ activation protocol on an Applied 430A peptide synthesizer. The sequence of the peptide is naturally found in Homo sapiens (Human). 参照: UniProt: Q13526, プロリルイソメラーゼ |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験 | タイプ: 2D NOESY |
-試料調製
詳細 | 内容: sample of 1mM Pin1 WW domain / 11mM tau ligand buffer of 50 mM deutered Tris-D2O, pH 6.4, 100 mM NaCl 溶媒系: 90% H2O/10% D2O |
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試料状態 | イオン強度: 100 mM NaCl / pH: 6.4 / 圧: ambient / 温度: 285 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: distance geometry simulated annealing / ソフトェア番号: 1 詳細: hybrid of distance geometry / simulated annealing protocol Minimization procedure using CVFF as force field | ||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 10 |