- PDB-1i85: CRYSTAL STRUCTURE OF THE CTLA-4/B7-2 COMPLEX -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 1i85
Title
CRYSTAL STRUCTURE OF THE CTLA-4/B7-2 COMPLEX
Components
CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED PROTEIN 4
T LYMPHOCYTE ACTIVATION ANTIGEN CD86
Keywords
IMMUNE SYSTEM / Ig V-type domain
Function / homology
Function and homology information
positive regulation of lymphotoxin A production / protein complex involved in cell adhesion / CD40 signaling pathway / negative regulation of regulatory T cell differentiation / positive regulation of T-helper 2 cell differentiation / activation of protein kinase C activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CD28 co-stimulation / CD28 dependent Vav1 pathway ...positive regulation of lymphotoxin A production / protein complex involved in cell adhesion / CD40 signaling pathway / negative regulation of regulatory T cell differentiation / positive regulation of T-helper 2 cell differentiation / activation of protein kinase C activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CD28 co-stimulation / CD28 dependent Vav1 pathway / positive regulation of immunoglobulin production / CTLA4 inhibitory signaling / positive regulation of interleukin-4 production / B cell activation / negative regulation of B cell proliferation / Interleukin-10 signaling / CD28 dependent PI3K/Akt signaling / : / centriolar satellite / coreceptor activity / negative regulation of T cell proliferation / positive regulation of T cell proliferation / T cell costimulation / positive regulation of interleukin-2 production / T cell activation / B cell receptor signaling pathway / positive regulation of non-canonical NF-kappaB signal transduction / Constitutive Signaling by Aberrant PI3K in Cancer / PIP3 activates AKT signaling / virus receptor activity / signaling receptor activity / T cell receptor signaling pathway / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / adaptive immune response / cellular response to lipopolysaccharide / receptor ligand activity / cell surface receptor signaling pathway / immune response / positive regulation of apoptotic process / external side of plasma membrane / DNA damage response / positive regulation of cell population proliferation / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / Golgi apparatus / cell surface / extracellular exosome / plasma membrane Similarity search - Function
The CTLA-4 homodimer is generated by Chain D and the translatonal symmetry mate of chain C (apply the operator x,y,z+1 to Chain C) / The repeating arrangement of CTLA-4 and B7-2 dimers is generated by applying the translational symmetry operation (x,y,z+/-N; where N ranges over all integers) to all chains in the asymmetric unit
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Components
#1: Antibody
TLYMPHOCYTEACTIVATIONANTIGENCD86 / B7-2
Mass: 12849.631 Da / Num. of mol.: 2 / Fragment: IG V-TYPE (RECEPTOR BINDING) DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CD86 / Plasmid: PET3A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P42081
#2: Protein
CYTOTOXICT-LYMPHOCYTE-ASSOCIATEDPROTEIN4 / CTLA-4
Mass: 13510.340 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CTLA4 / Plasmid: PET3A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P16410
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.59 Å3/Da / Density % sol: 52 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG20K, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystal grow
*PLUS
Details: drop consists of equal amounts of protein and reservoir solutions
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